Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:81355
Yang Y, et al., Wnt5a and Wnt5b exhibit distinct activities in coordinating chondrocyte proliferation and differentiation. Development. 2003 Mar;130(5):1003-15
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:2450642
|
Gene symbol:
|
Ihh
|
Gene name:
|
Indian hedgehog
|
Probe:
|
Ihh cDNA1
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
Visualized with:
|
Autoradiography
|
|
Results
|
Specimen
1C:
embryonic day 13.5
(more )
Specimen
1C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Formaldehyde
|
Embedding:
|
Paraffin
|
Specimen
2B, WT:
embryonic day 12.5
(more )
Specimen
2B, WT:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Formaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: arm |
Strong |
Regionally restricted |
2B WT Ihh
|
Strong expression in developing cartilage
|
Specimen
2B, Wnt5a -/-:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Wnt5atm1Amc/Wnt5atm1Amc
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Formaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: arm |
Absent |
|
2B Wnt5a -/- Ihh
|
Expression reduced to below detectable levels in cartilage.
|
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Wnt5atm1Amc/Wnt5atm1Amc
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Formaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: arm |
Weak |
|
|
Authors report weak expression in developing cartilage.
|
Specimen
2C, WT:
embryonic day 15.5
(more )
Specimen
2C, WT:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Formaldehyde
|
Embedding:
|
Paraffin
|
Specimen
2C, Wnt5a -/-:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Wnt5atm1Amc/Wnt5atm1Amc
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Formaldehyde
|
Embedding:
|
Paraffin
|
Specimen
5B, WT:
embryonic day 14.5
(more )
Specimen
5B, WT:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Formaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: forearm |
Present |
Regionally restricted |
5B WT Ihh
|
Expression in prehypertrophic chondrocytes.
|
Specimen
6A:
embryonic day 15.5
(more )
Specimen
6A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Formaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: humerus |
Present |
Regionally restricted |
6A Ihh
|
Expression in prehypertrophic chondrocytes.
|
Specimen
6C, WT:
embryonic day 15.5
(more )
Specimen
6C, WT:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Formaldehyde
|
Embedding:
|
Paraffin
|
|