Probe:	Tll cDNA1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS11: cardiogenic plate	Present	Spotted	4A 3rd row	Expression was localized to the anterior portion of the embryo, distributed symmetrically to either side of the neural groove in precardiac mesoderm.

Structure	Level	Pattern	Image	Note
TS11: cardiogenic plate	Present	Spotted	4B 3rd row	Expression was localized to the anterior portion of the embryo, distributed symmetrically to either side of the neural groove in precardiac mesoderm.

Structure	Level	Pattern	Image	Note
TS13: common atrial chamber endocardial tube	Strong		5A/B
TS13: extraembryonic vascular system	Present	Regionally restricted	5A/B	Expression was detected in the blood islands associated with extraembryonic membranes.
TS13: heart	Present	Regionally restricted	5A/B	Expression was detected in the endocardial layer of the heart tube.

Structure	Level	Pattern	Image	Note
TS13: bulbus cordis rostral half endocardial tube	Absent			Expression was absent from the endocardial lining of the bulbus cordis region of the primitive outflow tract.
TS13: outflow tract	Absent			Expression was absent from the endocardial lining of the bulbus cordis region of the primitive outflow tract.

Structure	Level	Pattern	Image	Note
TS13: sinus venosus left horn	Strong	Regionally restricted	5C/D	Expression was detected in the endocardial lining.
TS13: sinus venosus right horn	Strong	Regionally restricted	5C/D	Expression was detected in the endocardial lining.

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Strong	Regionally restricted	5E/F	Expression was strong in the midline dorsal aorta.
TS15: umbilical vein	Strong		5E/F
TS15: common atrial chamber endocardial lining	Present	Regionally restricted	5E/F	Expression was localized to the endocardial lining of the common atrial chamber overlying the dorsal mesocardium.
TS15: primitive ventricle cardiac muscle	Present	Regionally restricted	5E/F	Expression was detected in the inferior myocardial wall.

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Strong	Regionally restricted	6A/B	Expression was strong in the midline dorsal aorta.
TS17: cardinal vein	Strong		6A/B
TS17: umbilical vein	Strong		6A/B
TS17: outflow tract endocardial tube	Present	Single cells	6A/B

Structure	Level	Pattern	Image	Note
TS17: primitive ventricle cardiac muscle	Present	Regionally restricted	6C/D	Expression was detected in the inferior and superior myocardial walls of the common ventricular chamber.

Structure	Level	Pattern	Image	Note
TS17: septum primum	Strong	Regionally restricted	6E/F	Expression was strong in the dorsal component.
TS17: common atrial chamber left part endocardial lining	Strong		6E/F
TS17: common atrial chamber right part endocardial lining	Strong		6E/F
TS17: sinus venosus	Strong		6E/F
TS17: hepatic primordium	Present		6E/F

Structure	Level	Pattern	Image	Note
TS17: atrioventricular canal	Absent			Expression was absent from the endocardial cushion tissue.

Structure	Level	Pattern	Image	Note
TS19: aortico-pulmonary spiral septum	Present	Regionally restricted	7A/B	Expression was just discernable in the cranial ridge of mesenchymatous tissue and in the endocardial cushion tissue, but only where this tissue is juxtaposed to the surrounding myocardial cuff.
TS19: 6th arch artery	Present		7A/B
TS19: medial-nasal process	Present		7A/B
TS19: pericardium	Present		7A/B

Structure	Level	Pattern	Image	Note
TS19: interventricular septum cardiac muscle	Strong		7C/D
TS19: atrioventricular canal	Trace	Regionally restricted	7C/D	Expression was weak or absent from the endocardial cushion tissue.

Structure	Level	Pattern	Image	Note
TS19: interventricular septum cardiac muscle	Strong		7E/F
TS19: septum primum	Present	Patchy	7E/F

Structure	Level	Pattern	Image	Note
TS19: left atrium auricular region endocardial lining	Strong	Regionally restricted	7G/H	Expression was detected in the endocardial lining adjacent to the septum primum.
TS19: right atrium auricular region endocardial lining	Strong	Regionally restricted	7G/H	Expression was detected in the endocardial lining adjacent to the septum primum.
TS19: sclerotome condensation	Strong		7G/H
TS19: cardinal vein	Present		7G/H

Structure	Level	Pattern	Image	Note
TS20: interventricular septum cardiac muscle	Strong	Uniform	8A/B
TS20: aortico-pulmonary spiral septum	Strong	Regionally restricted	8A/B	Expression was strong in the cranial ridge of mesenchymatous tissue.

Structure	Level	Pattern	Image	Note
TS20: interventricular septum cardiac muscle	Strong		8C/D
TS20: aortico-pulmonary spiral septum	Strong	Regionally restricted	8C/D	Expression was detected in the endocardial cushion tissue.

Structure	Level	Pattern	Image	Note
TS20: atrioventricular endocardial cushion	Absent		8E/F
TS20: septum primum	Strong	Regionally restricted	8E/F	Expression was detected in the endocardial lining.
TS20: trachea mesenchyme	Present	Regionally restricted	8E/F	Expression was detected in the condensed mesenchyme.
TS20: cardiovascular system	Present		8E/F
TS20: vena cava	Strong		8E/F

Structure	Level	Pattern	Image	Note
TS20: cardinal vein	Strong

Structure	Level	Pattern	Image	Note
TS21: interventricular septum cardiac muscle	Present		8G	Expression is down-regulated at this stage relative to earlier stages.
TS21: septum primum	Strong		8G
TS21: endocardial tissue	Present		8G
TS21: left atrium auricular region endocardial lining	Present	Spotted	8G
TS21: right atrium auricular region endocardial lining	Present	Spotted	8G
TS21: left ventricle endocardial lining	Present	Spotted	8G	Expression is punctate in the trabeculations.
TS21: right ventricle endocardial lining	Present	Spotted	8G	Expression is punctate in the trabeculations.

Structure	Level	Pattern	Image	Note
TS20: aortico-pulmonary spiral septum	Strong	Regionally restricted	9C	Expression was strong in the endocardial cushion.
TS20: interventricular septum cardiac muscle	Strong		9C

Structure	Level	Pattern	Image	Note
TS21: aortico-pulmonary spiral septum	Present