Probe:	c21
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS11: node	Present		2B

Structure	Level	Pattern	Image	Note
TS11: node	Present		2D
TS11: notochordal process	Present		2D

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present	Regionally restricted	2H	Expression was detected ventrally.
TS12: future midbrain	Present	Regionally restricted	2H	Expression was detected ventrally.
TS12: primitive streak	Present	Regionally restricted	2H	Expression was detected in the node.

Structure	Level	Pattern	Image	Note
TS12: future midbrain	Present	Regionally restricted	2L , 2K	Expression was detected ventrolaterally.
TS12: future prosencephalon floor plate	Present	Regionally restricted	2L , 2K	Expression was detected ventrolaterally.
TS12: neural tube floor plate	Present		2L , 2K	Expression was detected in the floor plate down much of the neural axis.
TS12: notochord	Present		2K , 2L
TS12: foregut diverticulum	Present		2K , 2L
TS12: primitive streak	Present	Regionally restricted	2K , 2L	Expression was detected in the node.
TS12: future hindbrain	Present	Regionally restricted	2K , 2L	Expression was present in the floor plate.

Structure	Level	Pattern	Image	Note
TS13: notochord	Present	Regionally restricted	2P	Expression in the head process was lost.
TS13: primitive streak	Present	Regionally restricted	2P	Expression was detected in the node.
TS13: gut endoderm	Present	Regionally restricted	2P	Expression was detected in the caudal endoderm.

Structure	Level	Pattern	Image	Note
TS19: metencephalon floor plate	Present
TS19: myelencephalon floor plate	Present
TS19: neural tube floor plate	Present

Structure	Level	Pattern	Image	Note
TS14: gut	Present	Regionally restricted	2R	Expression was uniformly detected along the dorso-ventral axis of caudal endoderm.

Structure	Level	Pattern	Image	Note
TS15: branchial arch endoderm	Present	Regionally restricted	2T	Expression was detected in the pharyngeal endoderm.

Structure	Level	Pattern	Image	Note
TS12: future hindbrain neural ectoderm	Present	Regionally restricted	3B	Expression was detected ventrally.
TS12: notochord	Present		3B

Structure	Level	Pattern	Image	Note
TS12: future prosencephalon	Present	Regionally restricted	3D	Expression was detected ventrolaterally.
TS12: foregut diverticulum	Present		3D

Structure	Level	Pattern	Image	Note
TS13: notochord	Present	Regionally restricted	3F	Expression was absent from the head process.
TS13: gut endoderm	Present	Regionally restricted	3F	Expression was detected in the caudal endoderm.
TS13: neural tube floor plate	Present		3F

Structure	Level	Pattern	Image	Note
TS15: central nervous system	Present	Regionally restricted	7A	Expression was detected at the ventral midline.