Gene Expression Data
Assay Details
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Assay
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Reference:
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J:51129
Bierkamp C, et al., Desmosomal localization of beta-catenin in the skin of plakoglobin null-mutant mice. Development. 1999 Jan;126(2):371-81
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2449102
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Gene symbol:
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Dsp
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Gene name:
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desmoplakin
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Antibody:
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anti-Desmoplakin
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
1E:
embryonic day 17.5
(more  )
Specimen
1E:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: epidermis |
Present |
Spotted |
1E
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Expression was seen in the desmosomes.
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Specimen
1F:
(close )
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Genetic Background:
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either: (involves: 129 * 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6)
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Mutant Allele(s):
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Juptm1Kem/Juptm1Kem
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: epidermis |
Present |
Regionally restricted |
1F
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Expression was diffusely distributed over the cell membrane and in the cytoplasm.
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Specimen
5A:
embryonic day 17.5
(more )
Specimen
5A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: gut |
Present |
Regionally restricted |
5A
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Expression was present in the desmosomes, punctate at apical junctions and weak along the basolateral membranes.
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Specimen
5B:
(close )
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Genetic Background:
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either: (involves: 129 * 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6)
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Mutant Allele(s):
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Juptm1Kem/Juptm1Kem
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: gut |
Present |
Regionally restricted |
5B
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Expression was diffuse, greatly reduced and cytoplasmic in the entire villus as compared to wild type. The wild type apical staining pattern was still visible in the crypt cells.
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Analysis Tools