Gene Expression Data
Assay Details
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Assay
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Reference:
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J:80018
Przemeck GK, et al., Node and midline defects are associated with left-right development in Delta1 mutant embryos. Development. 2003 Jan;130(1):3-13
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2449016
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Gene symbol:
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Lefty2
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Gene name:
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left-right determination factor 2
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Probe:
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Lefty-2 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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In the paper, the authors referred to the Lefty2 gene as Leftb (and the Lefty1 gene as Ebaf.)
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Results
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Specimen
2F:
embryonic day 8.5
(more  )
Note: 4-7 somites.
Specimen
2F:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-7 somites.
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Specimen
Not Shown, +/-:
embryonic day 8.5; Dll1tm1Gos/Dll1 +
(more )
Note: 4-7 somites.
Specimen
Not Shown, +/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Dll1tm1Gos/Dll1+
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-7 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: lateral plate mesoderm |
Present |
Regionally restricted |
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Expression in the left lateral plate mesoderm.
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Specimen
2G:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Dll1tm1Gos/Dll1tm1Gos
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-7 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
2G
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Expression in this mutant was at the left side (5/16), the right side (3/16), bilateral (4/16 as shown here), or was not detected.
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Specimen
2H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Dll1tm1Gos/Dll1tm1Gos
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-7 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
2H
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Expression in this mutant was at the left side (5/16, shown here), the right side (3/16), bilateral (4/16), or was not detected.
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Analysis Tools