Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:80018
Przemeck GK, et al., Node and midline defects are associated with left-right development in Delta1 mutant embryos. Development. 2003 Jan;130(1):3-13
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:2449014
|
Gene symbol:
|
Hand1
|
Gene name:
|
heart and neural crest derivatives expressed 1
|
Probe:
|
Hand1 probe1
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
|
Results
|
Specimen
1B, +/+:
embryonic day 10.5
(more )
Specimen
1B, +/+:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: primitive ventricle |
Present |
Regionally restricted |
1B left
|
Expression in a large domain in the proampulla.
|
TS17: heart |
Present |
Regionally restricted |
1B left
|
Expression in a region of the metampulla (future right ventricle).
|
Specimen
1B, -/-:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
|
Mutant Allele(s):
|
Dll1tm1Gos/Dll1tm1Gos
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: heart |
Present |
Regionally restricted |
1B right
|
Expression in this mutant was detected in the appropriate position, although in reversed location. This mutant has a mirror-imaged morphology.
|
TS17: primitive ventricle |
Present |
Regionally restricted |
1B right
|
Expression in this mutant was detected in the appropriate position, although in reversed location. This mutant has a mirror-imaged morphology.
|
|