Probe:	pBS/ME1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS10: embryo ectoderm	Strong		1A/B
TS10: neural ectoderm	Strong	Regionally restricted	1A/B	Expression in the future headfold region.
TS10: embryo mesoderm	Present	Regionally restricted	1A/B	Expression in the embryonic mesoderm in the vicinity of the primitive streak.
TS10: embryo	Present	Regionally restricted	1A/B	Expression in the notochord plate.
TS10: amniotic fold	Present	Regionally restricted	1A/B	Expression in posterior amniotic fold.
TS10: extraembryonic component	Present	Regionally restricted	1A/B	Expression in amnion and chorion.
TS28: decidua	Absent		1A/B

Structure	Level	Pattern	Image	Note
TS10: embryo ectoderm	Strong		1C/D
TS10: neural ectoderm	Strong	Regionally restricted	1C/D	Expression in the future headfold region.
TS10: amniotic fold	Present	Regionally restricted	1C/D	Expression in posterior amniotic fold.
TS10: extraembryonic component	Present	Regionally restricted	1C/D	Expression in amnion and chorion.
TS28: decidua	Absent		1C/D

Structure	Level	Pattern	Image	Note
TS11: future brain neural fold	Very strong		1E/F
TS11: embryo mesoderm	Present	Regionally restricted	1E/F	Underlying the forming neural folds.
TS11: primitive streak	Absent		1E/F
TS11: allantois mesoderm	Present		1E/F
TS11: yolk sac mesoderm	Present		1E/F

Structure	Level	Pattern	Image	Note
TS12: anterior pro-rhombomere neural fold	Strong	Regionally restricted	2A/B	Expression in the proliferating and differentiating neuroepithelium.
TS12: future hindbrain neural fold	Strong	Regionally restricted	2A/B	Expression in the proliferating and differentiating neuroepithelium.
TS12: future midbrain neural fold	Strong	Regionally restricted	2A/B	Expression in the proliferating and differentiating neuroepithelium.
TS12: future prosencephalon neural fold	Strong	Regionally restricted	2A/B	Expression in the proliferating and differentiating neuroepithelium.
TS12: future spinal cord neural fold	Strong	Regionally restricted	2A/B	Expression in the proliferating and differentiating neuroepithelium.
TS12: posterior pro-rhombomere neural fold	Strong	Regionally restricted	2A/B	Expression in the proliferating and differentiating neuroepithelium.
TS12: head mesenchyme	Present	Regionally restricted	2A/B	Underlying the cranial neural folds.
TS12: trunk paraxial mesenchyme	Present		2A/B
TS12: amnion ectoderm	Absent		2A/B
TS12: amnion mesenchyme	Absent		2A/B

Structure	Level	Pattern	Image	Note
TS13: branchial arch	Strong		2C/D
TS13: head mesenchyme	Strong		2C/D
TS13: head mesenchyme derived from neural crest	Strong		2C/D
TS13: neural ectoderm	Strong		2C/D
TS13: notochord	Strong		2C/D
TS13: optic eminence	Strong		2C/D

Structure	Level	Pattern	Image	Note
TS14: future brain	Present	Regionally restricted	3A	In the forming neuroepithelium.
TS14: neural tube	Present	Regionally restricted	3A	Expression in the caudal neural tube as the neural folds were about to close.
TS14: head mesenchyme	Present	Regionally restricted	3A	In cephalic mesenchyme and around the optic vesicle and the otic vesicle.
TS14: 1st branchial arch mesenchyme	Present		3A
TS14: 2nd branchial arch mesenchyme	Present		3A

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventricular layer	Present	Graded	3B	A gradient in the ventricular zone along the dorsal ventral axis of the developing spinal cord.

Structure	Level	Pattern	Image	Note
TS20: medulla oblongata	Strong		4A
TS20: midbrain	Strong		4A
TS20: pons	Strong		4A
TS20: telencephalon ventricular layer	Strong		4A
TS20: cerebellum primordium	Present	Regionally restricted	4A	In rhombic lip cells and progenitor cells.
TS20: head mesenchyme	Present		4A

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex ventricular layer	Present		4C
TS23: head mesenchyme	Present	Regionally restricted	4C	Expression in the mesenchyme surrounding the nasal sinuses and teeth.
TS23: vibrissa	Present		4C

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Strong	Regionally restricted	5A	Expression in the subventricular zone and the rostral migratory pathway.
TS27: main olfactory bulb	Strong		5A

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Strong	Regionally restricted	5B	Expression in the subventricular zone and the rostral migratory pathway.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	5C	Expression in the subventricular zone.