Antibody:	Anti-smooth muscle alpha-Actin (clone 1A4)
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS23: lung	Present	Regionally restricted	5A	Expression was detected in the bronchial walls.
TS23: lung vascular element	Present	Regionally restricted	5A	Expression was detected in the walls.
TS23: lung mesenchyme	Present	Regionally restricted	5A	Expression was detected surrounding the proximal tubule but was absent from the mesenchyme surrounding the distal epithelium.

Structure	Level	Pattern	Image	Note
TS23: lung	Present	Regionally restricted	5B	Expression was detected in the bronchial walls.
TS23: lung mesenchyme	Present	Regionally restricted	5B	Expression was detected surrounding the proximal tubule but was absent from the mesenchyme surrounding the distal epithelium.
TS23: lung vascular element	Present	Regionally restricted	5B	Expression was detected in the walls.

Structure	Level	Pattern	Image	Note
TS23: lung	Present	Regionally restricted	5C	Expression was detected in the bronchial walls. Expression was the same in mutant and wild type.
TS23: lung mesenchyme	Present	Regionally restricted	5C	Expression was detected surrounding the proximal tubule but was absent from the mesenchyme surrounding the distal epithelium. Expression was the same in mutant and wild type.
TS23: lung vascular element	Present	Regionally restricted	5C	Expression was detected in the walls. Expression was the same in mutant and wild type.

Structure	Level	Pattern	Image	Note
TS23: lung	Present	Regionally restricted	5D	Expression was detected in the bronchial walls. Expression was the same in mutant and wild type.
TS23: lung mesenchyme	Present	Regionally restricted	5D	Expression was detected surrounding the proximal tubule but was absent from the mesenchyme surrounding the distal epithelium. Expression was the same in mutant and wild type.
TS23: lung vascular element	Present	Regionally restricted	5D	Expression was detected in the walls. Expression was the same in mutant and wild type.

Structure	Level	Pattern	Image	Note
TS27: bronchus	Present		5E
TS27: lung vascular element	Present	Regionally restricted	5E	Expression in large blood vessels, but absent from the capillaries.

Structure	Level	Pattern	Image	Note
TS27: bronchus	Present		5F
TS27: lung vascular element	Present	Regionally restricted	5F	Expression in large blood vessels, but absent from the capillaries.

Structure	Level	Pattern	Image	Note
TS28: bronchus	Present		5G
TS28: lung vascular element	Present	Regionally restricted	5G	Expression in large blood vessels, but absent from the capillaries.

Structure	Level	Pattern	Image	Note
TS28: bronchus	Present		5H
TS28: lung vascular element	Present	Regionally restricted	5H	Expression was detected in the large blood vessels and was upregulated in the arterioles relative to wild type but was absent from the capillaries.