Gene Expression Data
Assay Details
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Assay
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Reference:
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J:43752
Xiang M, et al., Requirement for Brn-3c in maturation and survival, but not in fate determination of inner ear hair cells. Development. 1998 Oct;125(20):3935-46
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2448677
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Gene symbol:
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Myo6
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Gene name:
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myosin VI
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Antibody:
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anti-myosin VI
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Detection system:
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Secondary antibody coupled to rhodamine
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Assay notes:
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Specimen was also immunolabeled for Pou4f3 (assay MGI:1289491).
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Results
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Specimen
3B:
embryonic day 15.5
(more  )
Note: Double labeled: red - Myo6; blue - Pou4f3 (assay MGI:1289491).
Specimen
3B:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Myo6; blue - Pou4f3 (assay MGI:1289491).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: inner ear vestibular component |
Present |
Regionally restricted |
3B
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Expression was restricted to the differentiated hair cells of the lumenal layer, where expression overlapped with Pou4f3 and was absent from the supporting cell layer.
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Specimen
Not Shown:
embryonic day 13.5
(more )
Note: Also stained for Pou4f3 (assay MGI:1289491).
Specimen
Not Shown:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Also stained for Pou4f3 (assay MGI:1289491).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: inner ear vestibular component |
Present |
Regionally restricted |
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Expression was restricted to the differentiated hair cells of the lumenal layer, where expression overlapped with Pou4f3 and was absent from the supporting cell layer.
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Specimen
Not Shown:
postnatal day 0
(more )
Note: Also stained for Pou4f3 (assay MGI:1289491).
Specimen
Not Shown:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Also stained for Pou4f3 (assay MGI:1289491).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: inner ear |
Present |
Regionally restricted |
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Expression was restricted to the differentiated hair cells of the lumenal layer in the vestibular sensory epithelia, where expression overlapped with Pou4f3 and was absent from the supporting cell layer.
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Analysis Tools