Tg(Cd4-CUL1-N252)1Ingh
Transgene Detail
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| Symbol: |
Tg(Cd4-CUL1-N252)1Ingh |
| Name: |
transgene insertion 1, Giorgio Inghirami |
| MGI ID: |
MGI:2447878 |
| Synonyms: |
Tg-Cul1-N252 |
| Transgene: |
Tg(Cd4-CUL1-N252)1Ingh Location: unknown
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| Alliance: |
Tg(Cd4-CUL1-N252)1Ingh page
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| Strain of Origin: |
Not Specified
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| Transgene Type: |
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Transgenic (Inserted expressed sequence) |
| Mutation: |
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Insertion
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Tg(Cd4-CUL1-N252)1Ingh expresses
1 gene
Transgene expresses:
| Organism |
Expressed Gene |
Homolog in Mouse |
Note |
| human |
CUL1 (8454) |
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Mutation details: The transgene consists of a mouse Cd4 promoter and a flag-tagged mutant human CUL1 sequence encoding 252 N-terminal residues. Mouse Cd4 regulatory sequences, including the minimal enhancer, minimal promoter, 70 bp of exon 1, and part of intron 1, were used to drive transgene expression in T lymphocytes. The Cd4 promoter lacks the silencer region and is transcriptionally active in both single- and double-positive T cells. Transgene expression was confirmed by Western blot analysis of thymocyte extracts from transgenic animals. The transgenic human protein was shown to associate with mouse Skp1 protein via immunoprecipitation immunoblot.
(J:80320)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Three transgenic lines (4, 10, and 20) were created. Highest expressing lines 10 and 20 were used for analysis.
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| Original: |
J:80320 Piva R, et al., In vivo interference with Skp1 function leads to genetic instability and neoplastic transformation. Mol Cell Biol. 2002 Dec;22(23):8375-87 |
| All: |
2 reference(s) |
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Analysis Tools
