Antibody:	Anti-HSPG-PRM
Detection system:	Secondary antibody coupled to Cy3

Structure	Level	Pattern	Image	Note
TS24: diencephalon ventricular layer	Present	Regionally restricted	7A	Expression was detected in the neural precursor cells lining the inner face of the ventricular zone.
TS24: brain	Present	Regionally restricted	7A	Expression was detected in the endothelial cells of blood vessels in the neuroepithelium.

Structure	Level	Pattern	Image	Note
TS24: telencephalon ventricular layer	Present		7C	Expression was detected in the neural precursor cells lining the inner face of the ventricular zone.
TS24: cardiovascular system	Present	Regionally restricted	7C	Expression was detected in the endothelial cells of blood vessels in the neuroepithelium.

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present	Graded	8A	Expression was detected in the neuroepithelial cells and was strongest close to the lumen.
TS14: neural tube floor plate	Strong		8A
TS14: future spinal cord	Strong	Regionally restricted	8A	Expression was detected in the basement membrane.
TS14: embryo mesenchyme	Present	Regionally restricted	8A	Expression was detected surrounding the spinal cord.

Structure	Level	Pattern	Image	Note
TS16: neural tube	Present	Graded	8B	Expression was detected in the neuroepithelial cells and was strongest close to the lumen.
TS16: neural tube floor plate	Present		8B
TS16: future spinal cord	Strong	Regionally restricted	8B	Expression was detected in the basement membrane.
TS16: embryo mesenchyme	Present	Regionally restricted	8B	Expression was detected surrounding the spinal cord.

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present	Regionally restricted	8D	Expression was detected in the neuroepithelial cells lining the lumen.
TS19: future spinal cord	Present	Regionally restricted	8D	Expression was detected in the dividing lateral wall/ventricular layer cells lining the central canal and the basement membrane.
TS19: embryo mesenchyme	Present	Regionally restricted	8D	Expression was detected surrounding the spinal cord.
TS19: cardiovascular system	Present	Regionally restricted	8D	Expression was detected in the endothelial cells of blood vessels of the neuroepithelium.

Structure	Level	Pattern	Image	Note
TS24: spinal cord	Present	Regionally restricted	8E , 8F	Expression was detected in the neuroepithelial cells lining the lumen. Expression was strong in the basement membrane.
TS24: dorsal root ganglion	Strong		8E , 8F
TS24: embryo mesenchyme	Present	Regionally restricted	8E , 8F	Expression was detected surrounding the spinal cord.

Structure	Level	Pattern	Image	Note
TS24: olfactory lobe	Present	Regionally restricted	9	Expression was detected in the processes of the olfactory epithelium, the basement membrane and was strongly detected in the extracellular matrix.

Structure	Level	Pattern	Image	Note
TS16: embryo	Present	Regionally restricted	10	Expression was detected in the basement membrane of various tissues and was strong in extracellular matrix.
TS16: embryo ectoderm	Present		10
TS16: embryo mesenchyme	Present		10
TS16: future hindbrain ventricular layer	Present	Regionally restricted	10	Expression was detected in the basement membrane and neuroepithelium.
TS16: future midbrain	Present	Regionally restricted	10	Expression was detected in the basement membrane and neuroepithelium.
TS16: trunk somite	Present	Graded	10	Expression was strongest in the dorsal aspect.
TS16: tail	Strong	Regionally restricted	10	Expression was detected in the neuroepithelial cells of the spinal cord lumen.
TS16: eye	Strong	Regionally restricted	10	Expression was detected in the eye capsule.
TS16: forelimb bud	Present	Regionally restricted	10	Expression was detected in the basement membrane.
TS16: lung	Present	Regionally restricted	10	Expression was detected in the basement membrane.
TS16: forelimb bud mesenchyme	Present		10
TS16: lung mesenchyme	Present		10
TS16: gut	Present	Regionally restricted	10	Expression was detected in the mesenchyme and the basement membrane.
TS16: cardiovascular system	Strong		10
TS16: heart	Strong		10