Gene Expression Data
Assay Details
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Assay
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Reference:
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J:36833
Joseph SJ, et al., A proteoglycan that activates fibroblast growth factors during early neuronal development is a perlecan variant. Development. 1996 Nov;122(11):3443-52
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2447875
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Gene symbol:
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Hspg2
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Gene name:
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perlecan (heparan sulfate proteoglycan 2)
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Antibody:
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Anti-HSPG-PRM
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
6A:
embryonic day 10.0
(more  )
Note: The plane of section was horizontal. Superimposed sections; green - Hspg2 (PRM antibody); red - Hspg2 (perlecan antibody; assay MGI:2447873).
Specimen
6A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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The plane of section was horizontal. Superimposed sections; green - Hspg2 (PRM antibody); red - Hspg2 (perlecan antibody; assay MGI:2447873).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: future spinal cord |
Present |
Regionally restricted |
6A
,
6C
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Expression was detected in the basement membrane and the neuroepithelial cells in the neuroepithelium.
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| TS16: embryo mesenchyme |
Present |
Regionally restricted |
6A
,
6C
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Expression was detected surrounding the spinal cord.
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Specimen
6D:
embryonic day 10.0
(more )
Note: The plane of section was horizontal. Superimposed sections; green - Hspg2 (PRM antibody); red - Hspg2 (perlecan antibody; assay MGI:2447873).
Specimen
6D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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The plane of section was horizontal. Superimposed sections; green - Hspg2 (PRM antibody); red - Hspg2 (perlecan antibody; assay MGI:2447873).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: neural tube |
Present |
Regionally restricted |
6D
,
6F
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Expression was detected in the neural precursor cells.
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| TS16: otocyst |
Present |
Regionally restricted |
6D
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6F
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Expression was detected in the neural precursor cells.
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| TS16: eye |
Present |
Regionally restricted |
6F
,
6D
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Expression was detected in the optic vesicle.
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Analysis Tools