Gene Expression Data
Assay Details
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Assay
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Reference:
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J:44765
Carpenter EM, et al., Targeted disruption of Hoxd-10 affects mouse hindlimb development. Development. 1997 Nov;124(22):4505-14
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2447682
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Gene symbol:
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Hoxd9
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Gene name:
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homeobox D9
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Probe:
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Hoxd9 probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
Not Shown:
embryonic day 12.5
(more  )
Specimen
Not Shown:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forelimb |
Present |
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| TS20: hindlimb |
Present |
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| TS20: vertebral cartilage condensation |
Present |
Graded |
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Labeling in the prevertebra was observed beginning at prevertebra 22 and continued caudally for approximately 8 segments.
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| TS20: spinal cord |
Strong |
Graded |
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Expression had an approximate anterior border in the spinal cord at the level of prevertebra 19. High levels of expression were maintained posteriorly through anterior lumbar segments and then decreased at more caudal levels.
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Specimen
Not Shown:
embryonic day 12.5; Hoxd10tm1Mrc/Hoxd10 +
(more )
Specimen
Not Shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Hoxd10tm1Mrc/Hoxd10+
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forelimb |
Present |
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Patterns of expression were not altered in heterozygous embryos.
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| TS20: hindlimb |
Present |
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Patterns of expression were not altered in heterozygous embryos.
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| TS20: vertebral cartilage condensation |
Present |
Graded |
|
Labeling in the prevertebra was observed beginning at prevertebra 22 and continued caudally for approximately 8 segments. Patterns of expression were not altered in heterozygous embryos.
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| TS20: spinal cord |
Strong |
Graded |
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Expression had an approximate anterior border in spinal cord at prevertebra 19 level. High levels of expression were maintained posteriorly through anterior lumbar segments then decreased at more caudal levels. Expression levels were unaltered in hets.
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Specimen
Not Shown:
(close )
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Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
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|
Mutant Allele(s):
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Hoxd10tm1Mrc/Hoxd10tm1Mrc
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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|
Fixation:
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4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forelimb |
Present |
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Patterns of expression were not altered in homozygous embryos.
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| TS20: hindlimb |
Present |
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Patterns of expression were not altered in homozygous embryos.
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| TS20: vertebral cartilage condensation |
Present |
Graded |
|
Labeling in the prevertebra was observed beginning at prevertebra 22 and continued caudally for approximately 8 segments. Patterns of expression were not altered in homozygous embryos.
|
| TS20: spinal cord |
Strong |
Graded |
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Expression had an approx. anterior border in the spinal cord at prevertebra 19 level. High levels of expression were maintained posteriorly through anterior lumbar segments and then decreased at more caudal levels. Expression was unaltered in mutants.
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