Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:78334
Liu W, et al., Sonic hedgehog regulates otic capsule chondrogenesis and inner ear development in the mouse embryo. Dev Biol. 2002 Aug 15;248(2):240-50
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:2447401
|
|
Gene symbol:
|
Pax2
|
|
Gene name:
|
paired box 2
|
|
Antibody:
|
anti Pax2 Z-RX2 (71-6000)
|
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
|
|
Assay notes:
|
The fixative was methacarn.
|
|
|
Results
|
Specimen
6A:
embryonic day 10.5
(more  )
Specimen
6A:
(close  )
|
Genetic Background:
|
either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: C57BL/6 * CBA)
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: otocyst |
Present |
Regionally restricted |
6A
|
Expression was detected in the ventromedial wall of the otic vesicle.
|
Specimen
6B:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Shhtm1Chg/Shhtm1Chg
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: otocyst |
Present |
Regionally restricted |
6B
|
Expression was markedly reduced in the ventromedial wall of the otic vesicle of the mutant relative to the wild-type.
|
|
Analysis Tools
