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Tg(GFAP-Hras1)1Agu
Transgene Detail
Summary
Symbol: Tg(GFAP-Hras1)1Agu
Name: transgene insertion 1, Abhijit Guha
MGI ID: MGI:2445441
Synonyms: RasD7
Transgene: Tg(GFAP-Hras1)1Agu  Location: unknown  
Alliance: Tg(GFAP-Hras1)1Agu page
Transgene
origin
Germline Transmission:  Earliest citation of germline transmission: J:69129
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Transgene
description
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
 
Tg(GFAP-Hras1)1Agu expresses 1 gene
 
Mutation details: The transgene consists of the human GFAP promoter fused to an Hras1 cDNA, an IRES-lacZ cassette, and a floxed PGK-neo cassette. The human GFAP promoter directs transgene expression in the astrocytes.The Hras1 sequence contains an HA tag at the amino terminus. Western blot analysis of astrocytes from transgenic animals confirmed cell-specific expression of the transgene. (J:69129)
Tumor Data
List all tumor models in MMHCdb carrying Tg(GFAP-Hras1)1Agu
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Notes
This transgenic is designated RasD7 and by Southern blot analysis was found to contain multiple transgene insertions at multiple sites. All animals from this line died between 10-14 days of age. 50% of mice of a second line, Tg(GFAP-Hras1)2Agu, died by 4 weeks of age.
References
Original:  J:69129 Ding H, et al., Astrocyte-specific expression of activated p21-ras results in malignant astrocytoma formation in a transgenic mouse model of human gliomas. Cancer Res. 2001 May 1;61(9):3826-36
All:  3 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory