Tg(Lck-ZAP70-Y315F)1Achn
Transgene Detail
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| Symbol: |
Tg(Lck-ZAP70-Y315F)1Achn |
| Name: |
transgene insertion 1, Andrew C Chan |
| MGI ID: |
MGI:2388333 |
| Synonyms: |
ZAP-70(Y315F) |
| Transgene: |
Tg(Lck-ZAP70-Y315F)1Achn Location: unknown
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| Alliance: |
Tg(Lck-ZAP70-Y315F)1Achn page
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| Transgene Type: |
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Transgenic (Inserted expressed sequence) |
| Mutation: |
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Insertion
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Tg(Lck-ZAP70-Y315F)1Achn expresses
1 gene
Transgene expresses:
| Organism |
Expressed Gene |
Homolog in Mouse |
Note |
| human |
ZAP70 (7535) |
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Transgene codes for a protein with a tyrosine to phenylalanine mutation. |
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Mutation details: The human ZAP70 gene carrying a tyrosine to phenylalanine substitution at position 315 (Y315F) was used as the transgene. The transgene is expressed in thymocytes under the control of mouse Lck promoter and contains a hemagglutinin epitope tag at the N-terminal end and a human growth hormone polyA tail. Transgene expression was verified by Western blot analysis.
(J:71098)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Two transgenic founders, line 1059 and line 1075, expressing comparable amounts of transgene product were created.
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| Original: |
J:71098 Gong Q, et al., Requirement for tyrosine residues 315 and 319 within zeta chain-associated protein 70 for T cell development. J Exp Med. 2001 Aug 20;194(4):507-18 |
| All: |
1 reference(s) |
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Analysis Tools
