Tg(KRT14-Prkcd)16Akv
Transgene Detail
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| Symbol: |
Tg(KRT14-Prkcd)16Akv |
| Name: |
transgene insertion 16, Ajit K Verma |
| MGI ID: |
MGI:2385694 |
| Synonyms: |
T7-PKC delta |
| Transgene: |
Tg(KRT14-Prkcd)16Akv Location: unknown
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| Alliance: |
Tg(KRT14-Prkcd)16Akv page
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| Transgene Type: |
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Transgenic (Inserted expressed sequence) |
| Mutation: |
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Insertion
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Tg(KRT14-Prkcd)16Akv expresses
1 gene
Transgene expresses:
| Organism |
Expressed Gene |
Note |
| mouse |
Prkcd (MGI:97598) |
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Mutation details: A mouse Prkcd cDNA tagged with 21 amino acids of bacteriophage T7 major coat protein at the N-terminus, and under the control of the human keratin 14 promoter, was used as the transgene. Human keratin 14 promoter sequences direct expression of the transgene in the basal cells of the epithelium. Western blot also detected expression of the transgene in stomach and thymus of transgenic animals. Expression of the transgene was 8X that of endogenous protein as determined by Western blot analysis. Kinase activity of the transgene product was confirmed by PKC immunocomplex kinase assay.
(J:58557)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Transgenic animals exhibit temporary hyperkeratosis of the tail beginning at 2-4 weeks of age.
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| Original: |
J:58557 Reddig PJ, et al., Transgenic mice overexpressing protein kinase Cdelta in the epidermis are resistant to skin tumor promotion by 12-O-tetradecanoylphorbol-13-acetate. Cancer Res. 1999 Nov 15;59(22):5710-8 |
| All: |
4 reference(s) |
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Analysis Tools
