Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Dlg2tm1Dsb
Name:	discs large MAGUK scaffold protein 2; targeted mutation 1, David S Bredt
MGI ID:	MGI:2388036
Synonyms:	Dlg2deltaE9, PSD93-
Gene:	Dlg2  Location: Chr7:90125880-92098455 bp, + strand  Genetic Position: Chr7, 51.07 cM
Alliance:	Dlg2tm1Dsb page

Germline Transmission:	Earliest citation of germline transmission: J:68924
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	129/Sv

Allele Type:    Targeted (Hypomorph, Modified isoform(s)) Mutations:    Insertion, Intragenic deletion   Mutation details: The exon encoding the second PDZ domain was replaced with a TK-neo cassette via homologous recombination. Northern blot analysis of brain RNA from homozygous mutant animals detected a faint mutant transcript of higher mobility, presumably an alternative splice product that skips the deleted exon. However, Western blot analysis of brain proteins using antibodies directed against a region N-terminal to the deleted exon did not detect protein product. Exon 9 is deleted. In knockout mice, the longer Dlg2gamma and DLG2alpha1 are not expressed in bone-marrow derived plasmacytoid dendritic cells. However, a shorter Dlg2n isoform is expressed in bone-marrow derived plasmacytoid dendritic cells and in brain (J:262606). (J:68924, J:262606)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

4 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Dlg2 Mutation:	61 strains or lines available

Original:	J:68924 McGee AW, et al., PSD-93 knock-out mice reveal that neuronal MAGUKs are not required for development or function of parallel fiber synapses in cerebellum. J Neurosci. 2001 May 1;21(9):3085-91
All:	20 reference(s)