Summary | Mutation origin | Mutation description | Expression | Phenotypes | Disease models | Tumor data | Find Mice (IMSR) | References

Symbol:	Mbd2tm1Bh
Name:	methyl-CpG binding domain protein 2; targeted mutation 1, Brian Hendrich
MGI ID:	MGI:2388002
Synonyms:	Mbd2-, Mbd2tm1.1Brd
Gene:	Mbd2  Location: Chr18:70701260-70759202 bp, + strand  Genetic Position: Chr18, 44.54 cM
Alliance:	Mbd2tm1Bh page

Germline Transmission:	Earliest citation of germline transmission: J:68356
Parent Cell Line:	E14TG2a (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Null/knockout, Reporter) Mutations:    Insertion, Intragenic deletion   Mutation details: Exon 2 was deleted and replaced with a promoter-less beta-geo cassette via homologous recombination. Northern blot confirmed the absence of normal transcript in homozygous mutant animals. RT-PCR analysis using primers to exon 1 and exon 3 detected a faint mutant read-through product. Western blot analysis of spleen and liver nuclear extracts from homozygous animals detected a small mutant protein presumed to be the translation product from exon 1, and would lack any functional domains. (J:68356)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

6 anatomical structure(s)

List all tumor models in MMHCdb carrying Mbd2tm1Bh

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Mbd2 Mutation:	21 strains or lines available

Original:	J:68356 Hendrich B, et al., Closely related proteins MBD2 and MBD3 play distinctive but interacting roles in mouse development. Genes Dev. 2001 Mar 15;15(6):710-23
All:	21 reference(s)