Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Myh10tm1Rsad
Name:	myosin, heavy polypeptide 10, non-muscle; targeted mutation 1, Robert S Adelstein
MGI ID:	MGI:2386439
Synonyms:	BdeltaI
Gene:	Myh10  Location: Chr11:68582385-68707458 bp, + strand  Genetic Position: Chr11, 41.95 cM, cytoband B3
Alliance:	Myh10tm1Rsad page

Germline Transmission:	Earliest citation of germline transmission: J:60967
Parent Cell Line:	J1 (ES Cell)
Strain of Origin:	129S4/SvJae

Allele Type:    Targeted (Hypomorph, Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: A 30 bp neural-specific exon referred to as N30 (also called the B1 insert) and the flanking introns were replaced with a PGK-neo cassette via homologous recombination. Deletion of the B1 insert was confirmed by RNA analysis of neural tissues. Northern blot of various tissues from homozygous mutant mice showed a significant decrease in gene expression. Western blot analysis showed a significant decrease in protein expression in both embryonic and adult mutant mice, including a decrease of 88% in the heart, of 65% in the brain, and of 64% in the lung. This is a mildly hypomorphic allele. (J:60967)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:	26 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Myh10 Mutation:	95 strains or lines available

Original:	J:60967 Uren D, et al., Gene dosage affects the cardiac and brain phenotype in nonmuscle myosin II-B-depleted mice. J Clin Invest. 2000 Mar;105(5):663-71
All:	2 reference(s)