Ghrl Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:76060 Liu YL, et al., Ghrelin gene expression is age-dependent and influenced by gender and the level of circulating IGF-I. Mol Cell Endocrinol. 2002 Mar 28;189(1-2):97-103
Assay type:	Northern blot
MGI Accession ID:	MGI:2386291
Gene symbol:	Ghrl
Gene name:	ghrelin

Probe:	rat ghrelin
Probe preparation:	Antisense labelled with P32 RNA
Visualized with:	Autoradiography
Assay notes:	4-8 experiments were done per time point.

Results

Image: 1

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
E18.5	E18.5	TS26: stomach	Present (a)	10-30 µg; total RNA	C57BL/6		Pooled
P3	P3	TS27: stomach	Present (b)	10-30 µg; total RNA	C57BL/6		Pooled
P7	P7	TS28: stomach	Present (c)	10-30 µg; total RNA	C57BL/6		Pooled
P14	P14	TS28: stomach	Present (d)	10-30 µg; total RNA	C57BL/6		Pooled
P21	P21	TS28: stomach	Present (e)	10-30 µg; total RNA	C57BL/6		Male
P60	P60	TS28: stomach	Present (f)	10-30 µg; total RNA	C57BL/6		Male
P180	P180	TS28: stomach	Present (g)	10-30 µg; total RNA	C57BL/6		Male
19 mo	P m 19	TS28: stomach	Present (h)	10-30 µg; total RNA	C57BL/6		Male

Notes:
(a) This time point has a relative expression level of 1.0.
(b) This time point has a relative expression level of 2.7.
(c) This time point has a relative expression level of 3.4.
(d) This time point has a relative expression level of 6.1.
(e) This time point has a relative expression level of 7.9. This is the time of peak expression.
(f) This time point has a relative expression level of 5.9.
(g) This time point has a relative expression level of 5.3.
(h) This time point has a relative expression level of 0.4. This was the lowest level of expression seen.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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