Gene Expression Data
Assay Details
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Assay
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Reference:
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J:63215
Schwitzgebel VM, et al., Expression of neurogenin3 reveals an islet cell precursor population in the pancreas. Development. 2000 Aug;127(16):3533-42
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2386025
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Gene symbol:
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Neurog3
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Gene name:
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neurogenin 3
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Antibody:
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anti-Neurogenin3
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
2A:
embryonic day 11.5
(more  )
Specimen
2A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: pancreas primordium |
Present |
Regionally restricted |
2A
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Expression in a few epithelial cells of the pancreatic bud.
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Specimen
2B:
embryonic day 12.5
(more )
Specimen
2B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2C:
embryonic day 15.5
(more )
Specimen
2C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: pancreas |
Present |
Regionally restricted |
2C
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Peak expression in nuclei of scattered ductal cells and occasional periductal cells.
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Specimen
2D:
embryonic day 18.5
(more )
Specimen
2D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: pancreas |
Present |
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2D
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Authors report expression is decreased at this stage.
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Specimen
5A:
embryonic day 15.5
(more )
Specimen
5A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5B:
(close )
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Genetic Background:
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involves: 129X1/SvJ
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Mutant Allele(s):
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Nkx2-2tm1Jlr/Nkx2-2tm1Jlr
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: pancreas |
Present |
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5B
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Authors report expression at levels equivalent to levels in wild-type littermates.
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Specimen
5C:
(close )
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Genetic Background:
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involves: 102/El * C3H/Rl
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Mutant Allele(s):
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Pax6Sey-Neu/Pax6Sey-Neu
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: pancreas |
Present |
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5C
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Authors report expression at levels equivalent to levels in wild-type littermates.
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Specimen
5D:
embryonic day 18.5
(more )
Specimen
5D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: pancreas |
Present |
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5D
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Authors report expression at levels equivalent to levels in wild-type littermates.
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Specimen
5E:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S2/SvPas)
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: pancreas |
Present |
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5E
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Authors report expression at levels equivalent to levels in wild-type littermates.
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Specimen
5F:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Neurod1tm1Mjts/Neurod1tm1Mjts
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: pancreas |
Present |
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5F
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Authors report expression at levels equivalent to levels in wild-type littermates.
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Analysis Tools