Probe:	Nspc1 probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: otocyst	Present		3A

Structure	Level	Pattern	Image	Note
TS17: genitourinary system	Present	Regionally restricted	3B	Expression is in the genital tubercule.

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present	Regionally restricted	3C	Expression is at the level of the anterior limb bud.

Structure	Level	Pattern	Image	Note
TS19: branchial arch	Strong		3D
TS19: heart	Strong		3D

Structure	Level	Pattern	Image	Note
TS19: genital tubercle	Strong		3E

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Strong		3F

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Strong		4A
TS19: nervous system	Present	Regionally restricted	4A	Expression is in the neural epithelium.

Structure	Level	Pattern	Image	Note
TS19: glossopharyngeal ganglion	Strong		4B
TS19: trigeminal ganglion	Strong		4B
TS19: vagus ganglion	Strong		4B

Structure	Level	Pattern	Image	Note
TS19: acoustic ganglion	Strong		4C
TS19: facial ganglion	Strong		4C
TS19: trigeminal ganglion	Strong		4C

Structure	Level	Pattern	Image	Note
TS19: genital tubercle	Present		4D

Structure	Level	Pattern	Image	Note
TS19: vagal nerve trunk	Present		4E

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Present		5A
TS21: metanephros	Present		5A
TS21: enteric nervous system	Present	Regionally restricted	5A	Expression is in the penian ganglion and enteric ganglia.
TS21: peripheral nervous system	Present	Regionally restricted	5A	Expression is in the penian ganglion and enteric ganglia.
TS21: parasympathetic nervous system	Present	Regionally restricted	5A	Expression is in the forming ganglia.
TS21: nervous system	Present	Regionally restricted	5A	Expression is in the neuroepithelium.
TS21: adrenal gland	Present	Regionally restricted	5A	Expression is in the forming adrenal gland.

Structure	Level	Pattern	Image	Note
TS21: trigeminal ganglion	Present		5B
TS21: vestibular ganglion	Present		5B
TS21: central nervous system ganglion	Present	Regionally restricted	5B	Expression is in the geniculate ganglion.

Structure	Level	Pattern	Image	Note
TS21: left atrium cardiac muscle	Present		5C
TS21: left ventricle cardiac muscle	Present		5C
TS21: right atrium cardiac muscle	Present		5C
TS21: right ventricle cardiac muscle	Present		5C

Structure	Level	Pattern	Image	Note
TS21: enteric nervous system	Present		5D	Expression is in the enteric ganglia.

Structure	Level	Pattern	Image	Note
TS21: adrenal gland	Present	Regionally restricted	5E	Expression is in the forming adrenal gland.

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Present		5F

Structure	Level	Pattern	Image	Note
TS21: genital tubercle of female	Present		5G	The strong staining is in the penian ganglion.

Structure	Level	Pattern	Image	Note
TS21: embryo	Present			The expression is widespread.