Probe:	Nedd4a cDNA4
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS21: forelimb pre-cartilage condensation	Present		1B	Expression in the cartilagenous elements of the fore-and hindlimb.
TS21: hindlimb pre-cartilage condensation	Present		1B	Expression in the cartilagenous elements of the fore-and hindlimb.

Structure	Level	Pattern	Image	Note
TS21: forelimb	Present	Regionally restricted	1D	Expression in the distal elements of the developing forelimb.
TS21: forelimb interdigital region	Absent		1D

Structure	Level	Pattern	Image	Note
TS22: forelimb	Present	Regionally restricted	2A	Expression in chondrocytes and at lower levels in condensed undifferentiated mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: forelimb	Present	Regionally restricted	2C	Expression in chondrocytes and at lower levels in condensed undifferentiated mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: forelimb digit	Strong	Regionally restricted	2D	Expression at the joint region.

Structure	Level	Pattern	Image	Note
TS22: forelimb	Strong	Regionally restricted	2E	Expression in the perichondrium, proliferating chondrocytes, and in pre-hypertrophic chondrocytes, but is undetectable in hypertrophic chondrocytes.

Structure	Level	Pattern	Image	Note
TS23: ulna	Present	Regionally restricted	2G	A decrease in expression as the chondrocytes become hypertrophic.

Structure	Level	Pattern	Image	Note
TS23: tarsus	Present	Regionally restricted	2I	Expression in proliferating chondrocytes.

Structure	Level	Pattern	Image	Note
TS23: tibia	Present	Regionally restricted	2K	Expression in proliferating and pre-hypertrophic chondrocytes, but is undetectable in hypertrophic chondrocytes.