About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:74052 Mayer A, et al., Developmental profile of Sry transcripts in mouse brain. Neurogenetics. 2000 Sep;3(1):25-30
Assay type: RT-PCR
MGI Accession ID: MGI:2384402
Gene symbol: Sry
Gene name: sex determining region of Chr Y
Probe: primer set s, 2a
Probe preparation: DNA
Visualized with: Ethidium bromide
Assay notes: In this assay, nested PCR was carried out, the first reaction with the outer primer set 1s/1a (MGI:2384069) resulting in a 567 bp product, the second reaction with the inner primer set s/2a (MGI:2384070) resulting in a 335 bp product. PCR products were further analyzed by Southern blots probed with Sry cDNA1 (MGI:2384076).
Results Image: 3A/B
 Sample Information Bands Other Sample Information
Lane AgeStructure 335 bp Amount Genetic BackgroundMutant Allele(s)Sex
E11, B E11.5 (a) TS19: brain Present Not Specified Not Specified Male
E13, B E13.5 (a) TS21: brain Present Not Specified Not Specified Male
E15, B E15.5 (a) TS23: brain Present Not Specified Not Specified Male
E17, B E17.5 (a) TS25: brain Present Not Specified Not Specified Male
E19, B E19.5 (a) TS26: brain Present Not Specified Not Specified Male
P14, M P14 TS28: midbrain Present (b) Not Specified Not Specified Male
P14, D P14 TS28: diencephalon Present (c) Not Specified Not Specified Male
P14, C P14 TS28: cerebral cortex Present (d) Not Specified Not Specified Male
P14, G P14 TS28: testis Present (e) Not Specified Not Specified Male
P30, M P30 TS28: midbrain Strong (f) Not Specified Not Specified Male
P30, D P30 TS28: diencephalon Present (e) Not Specified Not Specified Male
P30, C P30 TS28: cerebral cortex Present (e) Not Specified Not Specified Male
P30, G P30 TS28: testis Present (e) Not Specified Not Specified Male
P60, M P60 TS28: midbrain Present (g) Not Specified Not Specified Male
P60, D P60 TS28: diencephalon Present (h) Not Specified Not Specified Male
P60, C P60 TS28: cerebral cortex Present (h) Not Specified Not Specified Male
P60, G P60 TS28: testis Present (e) Not Specified Not Specified Male
Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) The transcript was found in 6 of 10 animals tested.
(c) The transcript was found in 2 of 9 animals tested.
(d) The transcript was found in 4 of 10 animals tested.
(e) The transcript was found in 5 of 5 animals tested.
(f) The transcript was found in 4 of 4 animals tested.
(g) The transcript was found in 2 of 10 animals tested.
(h) The transcript was found in 3 of 10 animals tested.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/14/2024
MGI 6.23 		The Jackson Laboratory