Summary | Mutation origin | Mutation description | Expression | Phenotypes | Tumor data | Find Mice (IMSR) | References

Symbol:	Prkdctm1Get
Name:	protein kinase, DNA activated, catalytic polypeptide; targeted mutation 1, Guillermo E Taccioli
MGI ID:	MGI:2383951
Synonyms:	DNA-PKcs-
Gene:	Prkdc  Location: Chr16:15455730-15660099 bp, + strand  Genetic Position: Chr16, 10.09 cM, cytoband B1
Alliance:	Prkdctm1Get page

Germline Transmission:	Earliest citation of germline transmission: J:50222
Parent Cell Line:	J1 (ES Cell)
Strain of Origin:	129S4/SvJae

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: The insertion of a neomycin selection cassette replaced 1 kb of sequence spanning two exons encoding amino acids 3860 through 3950. While RT-PCR analysis revealed the presence of truncated transcript in homozygous mutant mice, protein was undetected via Western blot analysis of thymic tissue. A kinase activity assay indicated functional ablation in homozygous mutant mice. (J:50222)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

6 anatomical structure(s)

List all tumor models in MMHCdb carrying Prkdctm1Get

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 3 strains available      Cell Lines: 0 lines available
Carrying any Prkdc Mutation:	408 strains or lines available

Original:	J:50222 Taccioli GE, et al., Targeted disruption of the catalytic subunit of the DNA-PK gene in mice confers severe combined immunodeficiency and radiosensitivity. Immunity. 1998 Sep;9(3):355-66
All:	17 reference(s)