Runx2tm1Gss
Targeted Allele Detail
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| Symbol: |
Runx2tm1Gss |
| Name: |
runt related transcription factor 2; targeted mutation 1, Gary S Stein |
| MGI ID: |
MGI:2183833 |
| Synonyms: |
Runx2 delta C |
| Gene: |
Runx2 Location: Chr17:44806873-45125518 bp, - strand Genetic Position: Chr17, 21.33 cM
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| Alliance: |
Runx2tm1Gss page
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Embryonic lethality in Runx2tm1Gss/Runx2tm1Gss and absence of clavicle in Runx2tm1Gss/Runx2+ mice
Show the 2 phenotype image(s) involving this allele.
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| Germline Transmission: |
Earliest citation of germline transmission:
J:70461
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| Parent Cell Line: |
AB2.2 (ES Cell)
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| Strain of Origin: |
129S7/SvEvBrd-Hprt1b-m2
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| Allele Type: |
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Targeted (Null/knockout) |
| Mutation: |
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Nucleotide substitutions
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Mutation details: A nonsense mutation was introduced after codon 376 of exon 8 resulting in deletion of the C-terminal region of the protein, which includes the nuclear matrix targeting signal (NMTS), and floxed TK-neo cassette was inserted into the intron preceding exon 8. Transient expression of cre recombinase in correctly targeted cells facilitated removal of the TK-neo cassette. RT-PCR analysis and Western blot confirmed the expression of the truncated gene product in homozygous mutant animals. Sequence analysis of the RT-PCR product confirmed the presence of the premature stop codon in exon 8.
(J:70461)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Runx2 Mutation: |
42 strains or lines available
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| Original: |
J:70461 Choi JY, et al., Subnuclear targeting of Runx/Cbfa/AML factors is essential for tissue-specific differentiation during embryonic development. Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8650-5 |
| All: |
11 reference(s) |
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Analysis Tools
