Gene Expression Data
Assay Details
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Assay
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Reference:
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J:77682
Zhu J, et al., beta8 integrins are required for vascular morphogenesis in mouse embryos. Development. 2002 Jun;129(12):2891-903
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2182613
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Gene symbol:
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Nes
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Gene name:
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nestin
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Antibody:
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clone RC2
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Detection system:
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Secondary antibody
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Results
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Specimen
5I:
embryonic day 10.5
(more  )
Specimen
5I:
(close  )
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Genetic Background:
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either: (involves: 129/Sv) or (involves: C57BL/6J)
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: brain |
Present |
Regionally restricted |
5I
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Radial glial organization in brain characterized by staining using the RC2 antibody.
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Specimen
5J:
(close )
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Genetic Background:
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either: (involves: 129/Sv) or (involves: 129/Sv * C57BL/6J)
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Mutant Allele(s):
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Itgb8tm1Lfr/Itgb8tm1Lfr
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: brain |
Present |
Regionally restricted |
5J
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Authors report that allignment of radial glial cell processes in the brain appear normal.
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Specimen
5K:
embryonic day 12.5
(more )
Specimen
5K:
(close )
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Genetic Background:
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either: (involves: 129/Sv) or (involves: C57BL/6J)
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
5K
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Radial glial processes extend in a parallel fashion from the ventricle towards the pial surface.
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Specimen
5L:
(close )
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Genetic Background:
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either: (involves: 129/Sv) or (involves: 129/Sv * C57BL/6J)
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Mutant Allele(s):
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Itgb8tm1Lfr/Itgb8tm1Lfr
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: ganglionic eminence |
Present |
Regionally restricted |
5L
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Radial glial cells are disorganized in the ganglionic eminence;.large cavitations with disruptions of radial glial processes are observed.
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Specimen
5M:
embryonic day 12.5
(more )
Specimen
5M:
(close )
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Genetic Background:
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either: (involves: 129/Sv) or (involves: C57BL/6J)
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: diencephalon |
Present |
Regionally restricted |
5M
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Radial glial processes extend in a parallel fashion from the ventricle towards the pial surface.
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Specimen
5N:
(close )
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Genetic Background:
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either: (involves: 129/Sv) or (involves: 129/Sv * C57BL/6J)
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Mutant Allele(s):
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Itgb8tm1Lfr/Itgb8tm1Lfr
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: diencephalon |
Present |
Regionally restricted |
5N
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Radial glial cells are disorganized in the diencephalon; processes appear tortuous and disconnected from the pial surface.
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Analysis Tools