Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Piwil1tm1Hfl
Name:	piwi-like RNA-mediated gene silencing 1; targeted mutation 1, Haifan Lin
MGI ID:	MGI:2182488
Synonyms:	miwinull
Gene:	Piwil1  Location: Chr5:128813135-128832538 bp, + strand  Genetic Position: Chr5, 67.86 cM
Alliance:	Piwil1tm1Hfl page

Germline Transmission:	Earliest citation of germline transmission: J:77015
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Allele Type:    Targeted (Null/knockout, Reporter) Mutations:    Insertion, Intragenic deletion   Mutation details: 15.5 kb of the gene spanning exons 3-22 were replaced with a loxP-flanked PGK-neo cassette preceded by a GFP reporter gene. The targeting event results in a GFP fusion product after the first 9 amino acids of the gene. Western blot and immunofluorescent microscopy of testis from homozygous mutant animals verified the absence of Piwil1 expression. (J:77015)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:	29 assay results 9 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Piwil1 Mutation:	28 strains or lines available

Original:	J:77015 Deng W, et al., miwi, a murine homolog of piwi, encodes a cytoplasmic protein essential for spermatogenesis. Dev Cell. 2002 Jun;2(6):819-30
All:	21 reference(s)