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Cyp7b1tm1Lth
Targeted Allele Detail
Summary
Symbol: Cyp7b1tm1Lth
Name: cytochrome P450, family 7, subfamily b, polypeptide 1; targeted mutation 1, Richard Lathe
MGI ID: MGI:2182328
Synonyms: Cyp7b1-
Gene: Cyp7b1  Location: Chr3:18126114-18297502 bp, - strand  Genetic Position: Chr3, 4.98 cM
Alliance: Cyp7b1tm1Lth page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:77685
Parent Cell Line:  E14TG2a (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion
 
Mutation detailsExon 2 was disrupted by the recombination of a construct containing IRES-lacZ and neo genes. Normal transcript was undetected by Northern blot analysis of homozygous mutant mice. Individual assays of various tissue homogenates showed a lack of DHEA hydroxylation, indicating the functional ablation of the encoded protein in homozygous mutant mice. The results obtained from beta-galactose staining differed from those of in situ hybridization. (J:77685)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 2 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Cyp7b1 Mutation:  32 strains or lines available
References
Original:  J:77685 Rose K, et al., Neurosteroid hydroxylase CYP7B: vivid reporter activity in dentate gyrus of gene-targeted mice and abolition of a widespread pathway of steroid and oxysterol hydroxylation. J Biol Chem. 2001 Jun 29;276(26):23937-44
All:  8 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory