Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Limk2tm1.1Tnak
Name:	LIM domain kinase 2; targeted mutation 1.1, Toshikazu Nakamura
MGI ID:	MGI:2180767
Synonyms:	Limk2-
Gene:	Limk2  Location: Chr11:3294256-3359189 bp, - strand  Genetic Position: Chr11, 2.34 cM
Alliance:	Limk2tm1.1Tnak page

Germline Transmission:	Earliest citation of germline transmission: J:73966
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Allele Type:    Targeted (Null/knockout) Mutation:    Intragenic deletion   Mutation details: This allele is a derivative of Limk2tm1Tnak. Cre-mediated recombination in vivo under control of the ACTB promoter excised the inserted neo cassette and exons 3, 4, 5, and 1t in the germline. Exons 3-5 encode the second LIM domain and part of the PDZ domain. Exon 1t encodes the initiation exon for the testis specific isoform of the protein. Western blot analysis did not detect protein in fibroblast cells derived from homozygous mutant embryos. Northern blot analysis of testis did not detect any mRNA in homozygous mutant mice. (J:73966)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:	61 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Limk2 Mutation:	74 strains or lines available

Original:	J:73966 Takahashi H, et al., Impaired spermatogenic ability of testicular germ cells in mice deficient in the LIM-kinase 2 gene. Dev Biol. 2002 Jan 15;241(2):259-72
All:	6 reference(s)