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Akt2tm1.1Mbb
Targeted Allele Detail
Summary
Symbol: Akt2tm1.1Mbb
Name: thymoma viral proto-oncogene 2; targeted mutation 1.1, Morris J Birnbaum
MGI ID: MGI:2158456
Synonyms: Akt2-, Akt2 KO, PKBbeta-
Gene: Akt2  Location: Chr7:27290977-27340251 bp, + strand  Genetic Position: Chr7, 15.94 cM, cytoband B1
Alliance: Akt2tm1.1Mbb page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:71491
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsThis allele is a derivative of Akt2tm1Mbb. Cre-mediated recombination in vivo under the control of a 6 kb 5'-flanking sequence from the Pou3f4 gene excised the floxed exons 4 and 5 in the germline. The excision of exons 4 and 5 results in a frameshift mutation that would lead to a premature termination even if the remaining exon 3 were to splice to exon 6. Exon 5 encodes the lysine residue necessary for catalytic activity. Western blot analyses using a polyclonal antibody did not detect protein in liver, muscle, and isolated adipocytes from homozygous mice. (J:71491)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Expression
In Mice Carrying this Mutation: 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation: 3 anatomical structure(s)
Tumor Data
List all tumor models in MMHCdb carrying Akt2tm1.1Mbb
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Akt2 Mutation:  51 strains or lines available
References
Original:  J:71491 Cho H, et al., Insulin resistance and a diabetes mellitus-like syndrome in mice lacking the protein kinase Akt2 (PKB beta). Science. 2001 Jun 1;292(5522):1728-31
All:  100 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/12/2024
MGI 6.23
The Jackson Laboratory