Gene Expression Data
Assay Details
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Assay
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Reference:
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J:73074
Bai CB, et al., Gli1 can rescue the in vivo function of Gli2. Development. 2001 Dec;128(24):5161-72
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2158410
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Gene symbol:
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Nkx2-2
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Gene name:
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NK2 homeobox 2
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Antibody:
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74.5A5
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Detection system:
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Secondary antibody coupled to Cy3
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Results
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Specimen
2C:
embryonic day 10.5
(more  )
Specimen
2C:
(close  )
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Genetic Background:
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involves: 129
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube lateral wall |
Present |
Regionally restricted |
2C
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Expression is present in the V3 interneurons adjacent to the floor plate.
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Specimen
2I:
(close )
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Genetic Background:
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either: (involves: 129S6/SvEvTac) or (involves: 129S6/SvEvTac * Black Swiss)
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Mutant Allele(s):
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Gli2tm2.1Alj/Gli2tm2.1Alj
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube lateral wall |
Present |
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2I
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Expression is present in the V3 interneurons adjacent to the floor plate. In the mutant the number of these interneurons is greatly reduced relative to the wild type.
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Specimen
6E:
embryonic day 10.5
(more )
Specimen
6E:
(close )
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Genetic Background:
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involves: 129
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube lateral wall |
Present |
Regionally restricted |
6E
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Expression is present in the interneurons in the ventralateral region.
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Specimen
6F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Absent |
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6F
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In the mutant the interneurons are lost.
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Specimen
6G:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129 * Black Swiss)
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Mutant Allele(s):
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Gli2tm2.1Alj/Gli2tm2.1Alj
Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Absent |
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6G
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In the mutant the interneurons are lost.
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Analysis Tools
