Gene Expression Data
Assay Details
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Assay
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Reference:
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J:73068
Spassky N, et al., Sonic hedgehog-dependent emergence of oligodendrocytes in the telencephalon: evidence for a source of oligodendrocytes in the olfactory bulb that is independent of PDGFRalpha signaling. Development. 2001 Dec;128(24):4993-5004
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2157536
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Gene symbol:
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Olig2
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Gene name:
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oligodendrocyte transcription factor 2
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Probe:
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pBRAH16
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Assay notes:
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The authors stated that they did in situ hybridizations using probes for Olig1 and Olig2 and saw similar patterns of expression; it is unclear which probe was used to stain the specimens pictured in Figure 6.
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Results
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Specimen
6A:
embryonic day 14.5
(more )
Specimen
6A:
(close )
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Genetic Background:
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CRL:OF1
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: medial ganglionic eminence |
Present |
Regionally restricted |
6A
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Mostly in the ventricular layer although there is some expression in scattered cells in the marginal layer.
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| TS22: olfactory lobe |
Absent |
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6A
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There is no expression in the olfactory bulb.
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Specimen
6B:
embryonic day 16.5
(more )
Specimen
6B:
(close )
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Genetic Background:
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CRL:OF1
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: olfactory lobe |
Present |
Single cells |
6B
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Expression is present in a few scattered cells of the olfactory bulb ventricular zone.
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Specimen
6C:
postnatal day 3
(more )
Specimen
6C:
(close )
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Genetic Background:
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CRL:OF1
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Age:
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postnatal day 3
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: main olfactory bulb |
Present |
Regionally restricted |
6C
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Expression is abundant mainly in the marginal layer and undetectable in the ventricular zone.
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