Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Trp53tm1Holl
Name:	transformation related protein 53; targeted mutation 1, Monica Hollstein
MGI ID:	MGI:2157167
Synonyms:	HUPKI, Hupki R72, p5372arg, p53hki, p53KI
Gene:	Trp53  Location: Chr11:69471185-69482699 bp, + strand  Genetic Position: Chr11, 42.83 cM, cytoband B2-C
Alliance:	Trp53tm1Holl page

Germline Transmission:	Earliest citation of germline transmission: J:68918
Parent Cell Line:	E14.1 (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Humanized sequence) Mutations:    Insertion, Intragenic deletion   Mutation details: Insertion of a loxP-flanked neomycin-thymidine kinase expression cassette replaced the mouse DNA-binding domain (exons 4-9) with the corresponding homologous segment of the normal human gene, leaving transcription under control of the endogenous promoter. Cre-mediated recombination in ES cells excised the neo-tk sequences. cDNA sequencing studies determined that transcripts from spleen of homozygous mutant mice carried the human sequence for exons 4-9, and that the gene is correctly spliced and transcribed. (J:68918)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

2 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 3 strains available      Cell Lines: 0 lines available
Carrying any Trp53 Mutation:	232 strains or lines available

Original:	J:68918 Luo JL, et al., Knock-in mice with a chimeric human/murine p53 gene develop normally and show wild-type p53 responses to DNA damaging agents: a new biomedical research tool. Oncogene. 2001 Jan 18;20(3):320-8
All:	18 reference(s)