Gene Expression Data
Assay Details
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Assay
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Reference:
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J:33133
Ahlgren U, et al., The morphogenesis of the pancreatic mesenchyme is uncoupled from that of the pancreatic epithelium in IPF1/ PDX1-deficient mice. Development. 1996 MAY;122(5):1409-1416
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2152862
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Gene symbol:
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Ins2
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Gene name:
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insulin II
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Antibody:
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anti Rat C-peptide Serum Guinea Pig
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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Antibody was generated against rat C-peptide II; reactivity with rat and mouse C-peptide, 100%; reactivity with rat C-peptide I, 20%; both Ins2 and Ins1 have been annotated.
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Results
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Specimen
5C:
embryonic day 10.5
(more  )
Specimen
5C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: pancreas primordium |
Present |
Single cells |
5C
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Expression in only a few cells in the dorsal pancreatic bud.
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Specimen
5D:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Pdx1tm1Ted/Pdx1tm1Ted
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: pancreas primordium |
Present |
Single cells |
5D
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Expression in only a few cells in the dorsal pancreatic bud.
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Specimen
5G:
embryonic day 13.5
(more )
Specimen
5G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: pancreas |
Present |
Regionally restricted |
5G
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Expression in an increased number of cells; expression in both dorsal and ventral pancreatic buds.
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Specimen
5H:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Pdx1tm1Ted/Pdx1tm1Ted
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: pancreas |
Absent |
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5H
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No insulin-expressing cells detectable at the level of the initial dorsal evagination.
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Analysis Tools
