Gene Expression Data
Assay Details
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Assay
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Reference:
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J:33133
Ahlgren U, et al., The morphogenesis of the pancreatic mesenchyme is uncoupled from that of the pancreatic epithelium in IPF1/ PDX1-deficient mice. Development. 1996 MAY;122(5):1409-1416
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2152856
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Gene symbol:
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Pdx1
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Gene name:
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pancreatic and duodenal homeobox 1
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Antibody:
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anti-IPF1
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
1A:
embryonic day 8.5
(more  )
Note: ~10 somites.
Specimen
1A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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~10 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: foregut-midgut junction |
Present |
Single cells |
1A
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Selective expression in a few cells in the dorsal and ventral gut epithelium form which the pancreatic buds evaginate; very little or no expression outside of the pancreatic buds.
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Specimen
1B:
embryonic day 9.0
(more )
Note: ~15 somites.
Specimen
1B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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~15 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: foregut |
Present |
Regionally restricted |
1B
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Expression clearly evident in both dorsal and ventral foregut endoderm.
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| TS14: foregut-midgut junction |
Present |
Regionally restricted |
1B
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Selective expression in developing pancreatic buds; very little or no expression outside of the pancreatic buds.
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Specimen
1C:
embryonic day 9.0
(more )
Note: ~20 somites.
Specimen
1C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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~20 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: foregut-midgut junction |
Strong |
Regionally restricted |
1C
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Strong expression in both pancreas primordia, which appear as protrusions of dorsal and ventral foregut; very little or no expression outside of the pancreatic buds.
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Specimen
1D:
embryonic day 9.5
(more )
Note: ~25 somites.
Specimen
1D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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~25 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: pancreas primordium |
Strong |
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1D
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High level of expression in developing pancreatic buds; very little or no expression outside of the pancreatic buds.
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Specimen
1E:
embryonic day 9.5
(more )
Note: ~25 somites. Double labeled: brown - Pdx1; dark blue - Shh (assay MGI:2152857).
Specimen
1E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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~25 somites. Double labeled: brown - Pdx1; dark blue - Shh (assay MGI:2152857).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: pancreas primordium |
Present |
Regionally restricted |
1E
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Expression in the dorsal and ventral epithelium; no expression in pancreatic mesenchyme.
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