Antibody:	anti-CRABP I
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	The authors indicate that they obtained identical results with two Crabp1 antibodies (see MGI:1340376 and MGI:4460229). Either antibody may have been used in this assay.

Structure	Level	Pattern	Image	Note
TS14: future brain	Present	Regionally restricted	5A	Staining was observed variably in the neuroepithelium of the cranial neural plate and neural folds.
TS14: future hindbrain	Present	Regionally restricted	5A	In the neural plate, labelling largely restricted to an area with a neuromeric (rhombomere-like) appearance.
TS14: neural crest	Strong		5A	Strong staining of neural crest cells at the tip of the neural folds.
TS14: mesenchyme derived from head mesoderm	Absent		5A	No specific staining in mesoderm.
TS14: surface ectoderm	Present	Regionally restricted	5A	Only local staining in ectoderm.
TS14: neural tube	Absent		5A	No staining in the closed neural tube in the midpart of the body axis.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present	Regionally restricted	5B	Specific staining of neural crest cells migrating from the dorsal part of the neural tube to the 1st visceral arch.
TS15: future midbrain	Present		5B

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component mesenchyme	Weak			Authors report that staining intensity was decreased.
TS17: 1st branchial arch maxillary component mesenchyme	Weak			Authors report that staining intensity was decreased.

Structure	Level	Pattern	Image	Note
TS17: 2nd branchial arch mesenchyme derived from neural crest	Strong		5C
TS17: 3rd branchial arch mesenchyme derived from neural crest	Strong		5C
TS17: 4th branchial arch mesenchyme derived from neural crest	Strong		5C
TS17: 6th branchial arch mesenchyme	Strong	Regionally restricted	5C
TS17: 2nd branchial arch mesenchyme derived from head mesoderm	Absent		5C
TS17: 3rd branchial arch mesenchyme derived from head mesoderm	Absent		5C
TS17: 4th branchial arch mesenchyme derived from head mesoderm	Absent		5C
TS17: branchial arch ectoderm	Absent		5C
TS17: branchial arch endoderm	Absent		5C

Structure	Level	Pattern	Image	Note
TS16: frontal process mesenchyme	Strong		5D
TS16: future midbrain	Strong	Regionally restricted	5D	Strong staining in cells in the outer layer of the midbrain roof, while the rest of the neuroepithelium of the midbrain showed no staining above background.
TS16: future midbrain roof plate	Strong	Regionally restricted	5D	Strong staining in cells in the outer layer of the midbrain roof, while the rest of the neuroepithelium of the midbrain showed no staining above background.
TS16: future forebrain	Absent		5D
TS16: neural tube	Strong		5D
TS16: dorsal root ganglion	Strong		5D
TS16: limb	Weak		5D

Structure	Level	Pattern	Image	Note
TS16: fronto-nasal process	Strong	Regionally restricted	5E	Strong staining in neural crest-derived frontonasal mesenchyme.
TS16: head mesenchyme	Strong	Regionally restricted	5E	Strong staining in the periocular area.
TS16: optic cup	Absent		5E
TS16: surface ectoderm	Absent		5E
TS16: mesenchyme derived from head mesoderm	Absent		5E

Structure	Level	Pattern	Image	Note
TS17: fronto-nasal process	Strong	Regionally restricted	6A	Strong staining in neural crest-derived frontonasal mesenchyme.
TS17: primitive ventricle	Absent		6D , 6A
TS17: forebrain	Absent		6A
TS17: midbrain floor plate	Absent		6A
TS17: midbrain lateral wall	Absent		6A
TS17: midbrain roof plate	Strong	Regionally restricted	6A	Strong staining in cells in the outer layer of the midbrain roof.
TS17: hindbrain	Present	Regionally restricted	6A , 6D	Less distinct pattern of staining in the hindbrain.
TS17: rhombomere 1	Present	Regionally restricted	6A , 6D	Marked ventrodorsal bands of staining localized to neuromeres (rhombomeres); stronger staining in neuromeres 4 and 6 than in neuromeres 1, 2, 3 and 5.
TS17: rhombomere 2	Present	Regionally restricted	6A , 6D	Marked ventrodorsal bands of staining localized to neuromeres (rhombomeres); stronger staining in neuromeres 4 and 6 than in neuromeres 1, 2, 3 and 5.
TS17: rhombomere 3	Present	Regionally restricted	6A , 6D	Marked ventrodorsal bands of staining localized to neuromeres (rhombomeres); stronger staining in neuromeres 4 and 6 than in neuromeres 1, 2, 3 and 5.
TS17: rhombomere 4	Present	Regionally restricted	6A , 6D	Marked ventrodorsal bands of staining localized to neuromeres (rhombomeres); stronger staining in neuromeres 4 and 6 than in neuromeres 1, 2, 3 and 5.
TS17: rhombomere 5	Present	Regionally restricted	6A , 6D	Marked ventrodorsal bands of staining localized to neuromeres (rhombomeres); stronger staining in neuromeres 4 and 6 than in neuromeres 1, 2, 3 and 5.
TS17: rhombomere 6	Present	Regionally restricted	6A , 6D	Marked ventrodorsal bands of staining localized to neuromeres (rhombomeres); stronger staining in neuromeres 4 and 6 than in neuromeres 1, 2, 3 and 5.

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Strong	Regionally restricted	6C	Strong staining of the dorsal roof of the hindbrain, expecially at the site of the anterior neuropore closure. Less distinct pattern of staining in the floor and walls; often stronger in the peripheral zone; stained cells are probably neurons.
TS15: 1st branchial arch	Present	Regionally restricted	6C	Neural crest-derived cells of the 1st visceral arch are stained.
TS15: head mesenchyme	Present	Single cells	6C	Scattered cells between and above the hindbrain and the otic vesicle.
TS15: otocyst	Weak		6C
TS15: surface ectoderm	Present	Regionally restricted	6C	Some staining is observed locally in ectoderm.
TS15: 1st branchial pouch endoderm	Absent		6C
TS15: mesenchyme derived from head mesoderm	Absent		6C

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	7A	Less distinct pattern of staining in the hindbrain.

Structure	Level	Pattern	Image	Note
TS16: dorsal root ganglion	Strong		8A
TS16: trunk mesenchyme derived from neural crest	Present	Single cells	8A	Staining in scattered cells, presumeably of neural crest origin, around the dorsal aorta and foregut.
TS16: neural tube lateral wall	Present	Single cells	8A	A few cells in the mantle layer of the neural tube also stained.
TS16: trunk somite	Absent		8A

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Single cells	8B	In the spinal cord, staining restricted to individual cells in the mantle layer (postmitotic early neurons), especially at the ventral horn.
TS17: neural tube	Present	Regionally restricted	8B	Strong staining in axons within the neural tube. No staining above background in proliferating neuroepithelial cells adjacent to the lumen.
TS17: spinal nerve	Strong	Regionally restricted	8B	Strong staining of motor neurons leaving the neural tube. Peripheral nerve bundles were stained.

Structure	Level	Pattern	Image	Note
TS14: trunk somite	Present	Regionally restricted		Expression in ventral portion of somites.

Structure	Level	Pattern	Image	Note
TS16: cranial ganglion	Strong	Regionally restricted		Strong staining in cranial nerves.