Crabp1 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:32533 Dencker L, et al., Localization of specific retinoid-binding sites and expression of cellular retinoic-acid-binding protein (CRABP) in the early mouse embryo. Development. 1990 Oct;110(2):343-52
Assay type:	Western blot
MGI Accession ID:	MGI:2152223
Gene symbol:	Crabp1
Gene name:	cellular retinoic acid binding protein I

Antibody:	anti-CRABP I
Detection system:	Secondary antibody coupled to alkaline phosphatase
Assay notes:	The authors indicate that they obtained identical results with two Crabp1 antibodies (see MGI:1340376 and MGI:4460229). Either antibody may have been used in this assay.

Results

Image: 4 ©

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	16 kDa (b)	Genetic Background	Mutant Allele(s)	Sex
10	E10.5 (a)	TS17: embryo	Present (c)	C57BL/6		Not Specified
11	E11.5 (a)	TS19: embryo	Present (c)	C57BL/6		Not Specified
12	E12.5 (a)	TS20: embryo	Present (c)	C57BL/6		Not Specified
13	E13.5 (a)	TS21: embryo	Present (c)	C57BL/6		Not Specified
14	E14.5 (a)	TS22: embryo	Weak (d)	C57BL/6		Not Specified
15	E15.5 (a)	TS23: embryo	Weak (d)	C57BL/6		Not Specified
16	E16.5 (a)	TS24: embryo	Ambiguous (e)	C57BL/6		Not Specified
17	E17.5 (a)	TS25: embryo	Ambiguous (e)	C57BL/6		Not Specified

Notes:
(a) Staging normalized so that morning of vaginal plug detection is E0.5.
(b) Antibody specifically recognizes a protein migrating close to 16-17 kDa.
(c) Authors report highest expression up to days 12 and 13.
(d) At days 14 to 17, expression was several-fold lower.
(e) Expression occurs below the level of detection at days 16 and 17 using the conditions in this experiment; authors state that this does not indicate lack of expression.

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