Probe:	Crabp-1 cDNA1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Probe length was reduced to an average of 150 nucleotides by limited alkaline hydrolysis.

Structure	Level	Pattern	Image	Note
TS13: branchial arch	Weak	Regionally restricted	1C CRABPI	Faint labelling of mesenchyme of pharyngeal arches; absent in ventral endoderm of pharyngeal pouches.
TS13: neural ectoderm	Strong	Regionally restricted	1C CRABPI
TS13: future prosencephalon	Absent		1C CRABPI
TS13: neural tube	Absent		1C CRABPI	Not expressed at post-cervical levels of trunk neural tube.
TS13: surface ectoderm	Absent		1C CRABPI
TS13: head mesenchyme	Weak	Regionally restricted	1C CRABPI	Faint labelling of mesenchyme of the frontonasal region.
TS13: head mesenchyme derived from neural crest	Strong	Regionally restricted	1C CRABPI	High levels in migrating neural crest cells and in neural crest cell-derived cranial ganglia.
TS13: migrating neural crest	Strong	Regionally restricted	1C CRABPI	High levels in migrating neural crest cells and in neural crest cell-derived cranial ganglia.
TS13: trigeminal neural crest	Strong		1C CRABPI
TS13: somatopleure	Present		1C CRABPI
TS13: splanchnopleure	Absent		1C CRABPI
TS13: foregut diverticulum endoderm	Absent		1C CRABPI	No transcripts detected in ventral endoderm of foregut and midgut.
TS13: midgut endoderm	Absent		1C CRABPI	No transcripts detected in ventral endoderm of foregut and midgut.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mandibular component mesenchyme	Present		1D CRABPI
TS15: head mesenchyme	Present	Regionally restricted	1D CRABPI	Transcripts in frontonasal mesenchyme.
TS15: head mesenchyme derived from neural crest	Present	Regionally restricted	1D CRABPI	Intense labelling in subpopulations of neural crest cells that later form the cranial ganglia.
TS15: trunk mesenchyme	Absent		1D CRABPI	Not detected in lateral mesenchyme.
TS15: foregut	Absent		1D CRABPI	Not detected in foregut and midgut endoderm.
TS15: midgut	Absent		1D CRABPI	Not detected in foregut and midgut endoderm.
TS15: otocyst	Absent		1D CRABPI
TS15: forelimb bud ectoderm	Absent		1D CRABPI	No labelling above background in ectoderm and apical ectodermal ridge (AER).
TS15: tail mesenchyme	Absent		1D CRABPI

Structure	Level	Pattern	Image	Note
TS12: future prosencephalon	Absent		2A CRABPI
TS12: future hindbrain	Present	Regionally restricted	2A CRABPI	Transcripts present in rhombomeres caudal to the preotic sulcus. Most abundant in a small portion of caudal hindbrain, immediately caudal to preotic sulcus.
TS12: posterior pro-rhombomere	Present		2A CRABPI
TS12: trunk unsegmented mesenchyme	Present	Regionally restricted	2A CRABPI	Transcripts present in undifferentiated presomitic mesenchyme.

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present	Regionally restricted	2B CRABPI	Most abundantly expressed in rhombomere B (the otic rhombomere); levels progressively decrease towards more rostral levels of neuroepithelium; lowest in midbrain and absent from forebrain regions.
TS12: posterior pro-rhombomere	Present		2B CRABPI
TS12: anterior pro-rhombomere	Present	Regionally restricted	2B CRABPI	Relatively intense signal at the basal side of the neuroepithelium rostral to the preotic sulcus.
TS12: otic placode	Present		2B CRABPI

Structure	Level	Pattern	Image	Note
TS13: future brain	Present	Regionally restricted	2C CRABPI	Lower levels at rostral levels of neuroepithelium
TS13: future midbrain	Weak		2C CRABPI	Lowest in midbrain region.
TS13: future prosencephalon	Absent		2C CRABPI
TS13: future hindbrain	Present	Regionally restricted	2C CRABPI	Caudal to the otic rhombomere, transcripts up to level of first occipital somites, including undifferentiated epiblast.
TS13: posterior pro-rhombomere	Strong		2C CRABPI	Most abundantly expressed in rhombomere B (the otic rhombomere).
TS13: trunk unsegmented mesenchyme	Present		2C CRABPI	Transcripts present in undifferentiated presomitic mesenchyme.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	2D CRABPI	Detected in all rhombomeres.
TS13: rhombomere 1	Weak		2D CRABPI	Lowest levels in rhombomeres 1 to 3.
TS13: rhombomere 2	Weak		2D CRABPI	Lowest levels in rhombomeres 1 to 3.
TS13: rhombomere 3	Weak		2D CRABPI	Lowest levels in rhombomeres 1 to 3.
TS13: rhombomere 4	Very strong		2D CRABPI	Highest levels in rhombomere 4.
TS13: rhombomere 5	Strong		2D CRABPI
TS13: future hindbrain posterior to rhombomere 5	Strong	Regionally restricted	2D CRABPI	High levels in rhombomere 6; intensity of signal progressively decreased in more caudal levels of neural epithelium.
TS13: rhombomere 1 neural crest	Present		2D CRABPI
TS13: rhombomere 2 neural crest	Present		2D CRABPI
TS13: future hindbrain posterior to rhombomere 5 neural crest	Present	Regionally restricted	2D CRABPI	Transcripts in neural crest cells adjacent to rhombomeres 1, 2 and 6 to 8.

Structure	Level	Pattern	Image	Note
TS14: facio-acoustic neural crest	Present		2E CRABPI
TS14: trigeminal neural crest	Present		2E CRABPI
TS14: head mesenchyme derived from neural crest	Present	Regionally restricted	2E CRABPI	Relatively weaker signal in neural crest cells that will contribute to cranial ganglia IX and X (derived from rhombomeres 6 and 7).
TS14: rhombomere	Present	Non-Uniform	2E CRABPI	All rhombomeres were labelled; most abundant in rhombomeres 4 to 6; intensity progressively decreased rostrally and caudally.
TS14: rhombomere 1	Present		2E CRABPI
TS14: rhombomere 2	Present		2E CRABPI
TS14: rhombomere 3	Present	Regionally restricted	2E CRABPI	Expression only in the most dorsal regions of epithelium of rhombomere 3.
TS14: rhombomere 4	Strong		2E CRABPI
TS14: rhombomere 5	Strong		2E CRABPI
TS14: rhombomere 6	Strong		2E CRABPI
TS14: otic pit	Absent		2E CRABPI

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	3A CRABPI	More abundant in distal regions, particularly in anterior regions; absent from more proximal central and dorsal mesenchyme.
TS17: neural tube floor plate	Absent		3A CRABPI	No transcripts in floor and roof plates of the neural tube.
TS17: neural tube roof plate	Absent		3A CRABPI	No transcripts in floor and roof plates of the neural tube.
TS17: neural tube lateral wall	Present	Regionally restricted	3A CRABPI	In the spinal cord, transcripts preferentially found in marginal and mantle layers.
TS17: cranial ganglion	Present		3A CRABPI

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	3B CRABPI	More abundant in distal regions, particularly in anterior regions; absent from more proximal central and dorsal mesenchyme.
TS17: neural tube floor plate	Absent		3B CRABPI	No transcripts in floor and roof plates of the neural tube.
TS17: neural tube roof plate	Absent		3B CRABPI	No transcripts in floor and roof plates of the neural tube.
TS17: neural tube lateral wall	Present	Regionally restricted	3B CRABPI	Transcripts preferentially found in marginal and mantle layers.
TS17: cranial ganglion	Present		3B CRABPI

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	3C CRABPI	More abundant in distal regions, particularly in anterior regions; absent from more proximal central and dorsal mesenchyme.
TS17: neural tube floor plate	Absent		3C CRABPI	No transcripts in floor and roof plates of the neural tube.
TS17: neural tube roof plate	Absent		3C CRABPI	No transcripts in floor and roof plates of the neural tube.
TS17: neural tube lateral wall	Present	Regionally restricted	3C CRABPI	Transcripts preferentially found in marginal and mantle layers.
TS17: cranial ganglion	Present		3C CRABPI

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	3D CRABPI	More abundant in distal regions, particularly in anterior regions; absent from more proximal central and dorsal mesenchyme.
TS17: neural tube floor plate	Absent		3D CRABPI	No transcripts in floor and roof plates of the neural tube.
TS17: neural tube roof plate	Absent		3D CRABPI	No transcripts in floor and roof plates of the neural tube.
TS17: neural tube lateral wall	Present	Regionally restricted	3D CRABPI	Transcripts preferentially found in marginal and mantle layers.
TS17: cranial ganglion	Present		3D CRABPI

Structure	Level	Pattern	Image	Note
TS21: hindlimb	Present	Regionally restricted	4B CRABPI	Expression in distal mesenchyme underlying the AER at the tip of digits; absent from cartilage and developing muscles; high levels in parts of perichondrium and mesenchyme.

Structure	Level	Pattern	Image	Note
TS15: surface ectoderm	Absent		5A CRABPI
TS15: trunk dermomyotome	Present	Regionally restricted	5A CRABPI	Transcripts low in somites and their derivatives; restricted to a small portion of the dermamyotome; myotomic portions of somites are more intensely labelled.
TS15: future forebrain	Absent		5A CRABPI
TS15: future midbrain	Present	Regionally restricted	5A CRABPI	Labelling of mesencephalon restricted to outermost cells.
TS15: neural tube lateral wall	Present	Regionally restricted	5A CRABPI	Transcripts preferentially found in marginal and mantle layers. There are no transcripts in floor and roof plates of the neural tube.
TS15: spinal ganglion	Present		5A CRABPI
TS15: olfactory placode	Absent		5A CRABPI	No expression in olfactory placodes.

Structure	Level	Pattern	Image	Note
TS15: surface ectoderm	Absent		5B CRABPI
TS15: forelimb bud mesenchyme	Present		5B CRABPI
TS15: trunk dermomyotome	Present	Regionally restricted	5B CRABPI	Myotomic portions of somites are more intensely labelled.
TS15: future forebrain	Absent		5B CRABPI
TS15: future midbrain	Present	Regionally restricted	5B CRABPI	Labelling of mesencephalon restricted to outermost cells.
TS15: neural tube lateral wall	Present	Regionally restricted	5B CRABPI	In the spinal cord, transcripts preferentially found in marginal and mantle layers. There are no transcripts in floor and roof plates of the neural tube.
TS15: spinal ganglion	Present		5B CRABPI
TS15: olfactory placode	Absent		5B CRABPI	No expression in olfactory placodes.

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Present	Regionally restricted	5C CRABPI	Particularly abundant in the distal portion of frontonasal and pharyngeal arch mesenchyme.
TS17: head mesenchyme	Present	Regionally restricted	5C CRABPI	Particularly abundant in the distal portion of frontonasal and pharyngeal arch mesenchyme.
TS17: forelimb bud mesenchyme	Present	Regionally restricted	5C CRABPI	More abundant in distal regions of limb buds; particularly in anterior regions, absent from more proximal central and dorsal mesenchyme.
TS17: hindlimb bud mesenchyme	Present	Uniform	5C CRABPI
TS17: neural tube lateral wall	Present	Regionally restricted	5C CRABPI	Transcripts preferentially found in marginal and mantle layers. There are no transcripts in floor and roof plates of the neural tube.
TS17: spinal ganglion	Present	Regionally restricted	5C CRABPI
TS17: stomach mesenchyme	Present	Regionally restricted	5C CRABPI	Labelling in mesenchyme of stomach close to dorsal mesentery.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Absent		6CRABPI
TS20: telencephalon	Absent		6CRABPI
TS20: hindbrain	Present	Regionally restricted	6CRABPI	Some labelling in mantle layer of metencephalon and myelencephalon.
TS20: metencephalon	Present	Regionally restricted	6CRABPI	Some labelling in mantle layer of metencephalon and myelencephalon.
TS20: midbrain	Present	Regionally restricted	6CRABPI	Only outermost cells of mesencephalon appeared to contain transcripts.
TS20: spinal ganglion	Absent		6CRABPI
TS20: fronto-nasal process	Strong	Regionally restricted	6CRABPI	Present in distal frontonasal mesenchyme.
TS20: mandibular process	Strong		6CRABPI
TS20: tongue	Strong		6CRABPI
TS20: liver	Absent		6CRABPI
TS20: genital tubercle	Present		6CRABPI
TS20: metanephros	Present		6CRABPI

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	7A CRABPI	Some labelling in mantle layer of metencephalon and myelencephalon.
TS20: metencephalon	Present	Regionally restricted	7A CRABPI	Some labelling in mantle layer of metencephalon and myelencephalon.
TS20: fronto-nasal process	Strong	Regionally restricted	7A CRABPI	Present in distal frontonasal mesenchyme.
TS20: thymus primordium	Absent		7A CRABPI
TS20: mandibular process	Strong		7A CRABPI
TS20: tongue	Strong		7A CRABPI

Structure	Level	Pattern	Image	Note
TS21: nose	Present	Regionally restricted	7B CRABPI	Present in distal frontonasal mesenchyme.
TS21: thymus primordium	Absent		7B CRABPI
TS21: mandible	Present		7B CRABPI
TS21: tongue	Present		7B CRABPI

Structure	Level	Pattern	Image	Note
TS17: spinal ganglion	Present		7C CRABPI
TS17: mesonephros mesenchyme	Present	Regionally restricted	7C CRABPI	Transcripts restricted to the dorsal mesonephric mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: gut epithelium	Absent		7D CRABPI
TS20: mesenchyme of metanephros	Present	Regionally restricted	7D CRABPI	Expression in mesenchymal cells surrounding the developing metanephros.
TS20: genital tubercle	Present	Regionally restricted	7D CRABPI	Labelling in periphery of genital tubercle mesenchyme; not present in the core mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: mesenchyme of metanephros	Present	Regionally restricted	7E CRABPI	Present only in stromal cells of the kidney.

Structure	Level	Pattern	Image	Note
TS17: Rathke's pouch	Absent		9A CRABPI
TS17: future adenohypophysis	Absent		9A CRABPI

Structure	Level	Pattern	Image	Note
TS20: neurohypophysis infundibulum	Absent		9B CRABPI
TS20: remnant of Rathke's pouch	Absent		9B CRABPI

Structure	Level	Pattern	Image	Note
TS21: alar columns	Present	Regionally restricted	9C CRABPI	Transcripts are preferentially found in the alar plate.
TS21: spinal cord floor plate	Absent		9C CRABPI
TS21: spinal cord roof plate	Absent		9C CRABPI
TS21: spinal cord	Present	Regionally restricted	9C CRABPI	Transcripts are preferentially found in the alar plate. No transcripts in neuroepithelial layer or the floor and roof plates.
TS21: spinal ganglion	Absent		9C CRABPI

Structure	Level	Pattern	Image	Note
TS21: neural retinal epithelium	Present	Regionally restricted	10A CRABPI	Labelling restricted to central area of retina.

Structure	Level	Pattern	Image	Note
TS21: nasal cavity epithelium	Present	Regionally restricted	10B CRABPI	Transcripts restricted to olfactory epithelium.
TS21: olfactory epithelium	Present	Regionally restricted	10B CRABPI	Preferentially expresssed in the middle layer.

Structure	Level	Pattern	Image	Note
TS21: dermis	Present		10C CRABPI
TS21: epidermis	Absent		10C CRABPI
TS21: vibrissa dermal component	Present		10C CRABPI

Structure	Level	Pattern	Image	Note
TS13: anterior pro-rhombomere neural crest	Present			Labelled neural crest cells migrating from neuroepithelium rostral to preotic sulcus to invade the first pharyngeal arch.
TS13: posterior pro-rhombomere neural crest	Strong			High levels in neural crest cells migrating from levels immediately rostral to the otic placode.
TS13: migrating neural crest	Present

Structure	Level	Pattern	Image	Note
TS17: surface ectoderm	Absent
TS17: nasal pit	Absent			No expression in olfactory placodes.
TS17: lung	Absent			No labelling above background in lung epithelium.

Structure	Level	Pattern	Image	Note
TS20: limb	Present	Regionally restricted		Restricted to the periphery.; absent from precartilage condensations and differentiated cartilage.
TS20: neurohypophysis infundibulum	Absent
TS20: remnant of Rathke's pouch	Absent
TS20: lung	Absent			No labelling above background in lung epithelium.

Structure	Level	Pattern	Image	Note
TS21: neurohypophysis infundibulum	Absent
TS21: remnant of Rathke's pouch	Absent
TS21: corpus striatum	Present
TS21: lung	Absent			No labelling above background in lung epithelium.

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present	Regionally restricted		Transcripts in hippocampus.
TS22: corpus striatum	Present
TS22: hindbrain meninges	Absent			Not expressed in meninges covering the brainstem.
TS22: medulla oblongata	Present
TS22: pons	Present
TS22: spinal ganglion	Present
TS22: lung	Absent			No labelling above background in lung epithelium.
TS22: limb	Present	Regionally restricted		Transcripts in connective tissues (tendons, ligaments and perichondrium) of the limb.