Gene Expression Data
Assay Details
Assay
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Reference:
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J:71240
Feiner L, et al., Targeted disruption of semaphorin 3C leads to persistent truncus arteriosus and aortic arch interruption. Development. 2001 Aug;128(16):3061-70
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:2150711
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Gene symbol:
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Sema3c
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Gene name:
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sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C
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Probe:
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primer set 2
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Probe preparation:
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Double stranded DNA
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Assay notes:
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Primer set 1 (blue; MGI:2149810) amplifies a region of the mRNA 5' of the targeted region, primer set 2 (red: MGI:2149812) a region 3' of the targeted region, and primer set 3 (green: MGI:2149814) a region internal to the targetedregion.
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Results
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Mol. Wt. Marker Lane |
E12.5
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TS20: embryo
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Present
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2.00 µg; total RNA |
CD-1 |
| Not Specified |
E12.5
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TS20: embryo
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Present
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2.00 µg; total RNA |
involves: 129P2/OlaHsd * CD-1 |
Sema3ctm1.1Jra/Sema3ctm1.1Jra | Not Specified |
Other lane(s) in blot use different probe(s) |
Mol. Wt. Marker Lane |
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