Gene Expression Data
Assay Details
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Assay
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Reference:
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J:64588
Toresson H, et al., Genetic control of dorsal-ventral identity in the telencephalon: opposing roles for pax6 and gsh2. Development. 2000 Oct;127(20):4361-71
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2150342
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Gene symbol:
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Pax6
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Gene name:
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paired box 6
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Probe:
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Pax6 probe10
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5A:
embryonic day 12.5
(more  )
Specimen
5A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
5A
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vHighest levels close to the border of GSH2 expression.
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Specimen
5E:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
5E
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Expression of mutated Pax6 transcript in its normal domain; downregulated in cortical ventricular zone (VZ), in the lateral-most part of the lateral ganglionic eminence (LGE), specifically within the domain of ectopic GSH2 expression.
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| TS20: lateral ganglionic eminence |
Present |
Regionally restricted |
5E
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Expression was downregulated in cortical ventricular zone (VZ), in the lateral-most part of the lateral ganglionic eminence (LGE), specifically within the domain of ectopic GSH2 expression.
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Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Gsx2tm1Ssp/Gsx2tm1Ssp
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
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Expression of mutated Pax6 transcript throughout the LGE.
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Specimen
6D:
embryonic day 14.0
(more )
Specimen
6D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6I:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: telencephalon |
Present |
Regionally restricted |
6I
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Mutated Pax6 transcript detected in its normal domain; significantly downregulated in lateral cortical VZ, specifically within the domain of ectopic GSH2 expression.
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Specimen
7A:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Gsx2tm1Ssp/Gsx2tm1Ssp
Pax6Sey/Pax6Sey
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
7A
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Expression of mutated Pax6 transcript at wild-type levels throughout the LGE.
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Specimen
7G:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: telencephalon |
Present |
Regionally restricted |
7G
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Downregulation of the mutated Pax6 transcript in cortical VZ.
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Specimen
7H:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Gsx2tm1Ssp/Gsx2tm1Ssp
Pax6Sey/Pax6Sey
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: telencephalon |
Present |
Regionally restricted |
7H
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Expression in cortical progenitors at wild-type levels.
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Analysis Tools