Summary | Mutation origin | Mutation description | Expression | Phenotypes | Disease models | Find Mice (IMSR) | References

Symbol: Nos3tm1Plh Name: nitric oxide synthase 3, endothelial cell; targeted mutation 1, Paul L Huang MGI ID: MGI:2150145 Synonyms: eNOS-, eNOS KO, NOSIII- Gene: Nos3  Location: Chr5:24569808-24589472 bp, + strand  Genetic Position: Chr5, 11.32 cM Alliance: Nos3tm1Plh page

Renal tubular apoptosis, regeneration, glomerulosclerosis and glomerular thrombus formation in Nos1tm1Plh/Nos1tm1Plh Nos2tm1Mrl/Nos2tm1Mrl Nos3tm1Plh/Nos3tm1Plh mice Show the 1 phenotype image(s) involving this allele.

Germline Transmission:	Earliest citation of germline transmission: J:28778
Parent Cell Line:	J1 (ES Cell)
Strain of Origin:	129S4/SvJae

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: A neomycin cassette replaced 3 exons that encode the NADPH ribose and adenine binding sites of the protein (amino acids 1010 - 1144). Western blots of protein extracts of brain, heart, lung, and aorta/vena cava from homozygous mutant mice showed no detectable encoded protein. (J:28778) Inheritance:    Dominant

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	28 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Nos3 Mutation:	56 strains or lines available

Original:	J:28778 Huang PL, et al., Hypertension in mice lacking the gene for endothelial nitric oxide synthase [see comments]. Nature. 1995 Sep 21;377(6546):239-42
All:	128 reference(s)