Gene Expression Data
Assay Details
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Assay
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Reference:
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J:62985
Helmbacher F, et al., Targeting of the EphA4 tyrosine kinase receptor affects dorsal/ventral pathfinding of limb motor axons. Development. 2000 Aug;127(15):3313-24
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2149749
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Gene symbol:
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Nefm
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Gene name:
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neurofilament, medium polypeptide
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Results
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Specimen
3A:
embryonic day 11.5
(more  )
Specimen
3A:
(close  )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Dent's Fixative
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: spinal nerve plexus |
Present |
Regionally restricted |
3A
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Expression in peroneal axons emerging from the sciatic plexus.
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Specimen
5C:
embryonic day 11.5
(more )
Specimen
5C:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Dent's Fixative
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: segmental spinal nerve |
Present |
Regionally restricted |
5C
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At the level of sciatic plexus, expression in dorsal and ventral bundles of axons.
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Specimen
5E:
embryonic day 11.5
(more )
Specimen
5E:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Dent's Fixative
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Specimen
5H:
embryonic day 11.5
(more )
Specimen
5H:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Dent's Fixative
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: segmental spinal nerve |
Present |
Regionally restricted |
5H
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At the level of sciatic plexus, expression in dorsal and ventral bundles of axons.
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Specimen
5I:
embryonic day 11.5
(more )
Specimen
5I:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Dent's Fixative
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: segmental spinal nerve |
Present |
Regionally restricted |
5I
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At the level of sciatic plexus, expression in dorsal and ventral bundles of axons.
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Specimen
6B,D:
embryonic day 11.5; Epha4tm1Pch/Epha4 +
(more )
Note: Expression detected by immunofluorescence.
Specimen
6B,D:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Mutant Allele(s):
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Epha4tm1Pch/Epha4+
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Dent's Fixative
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Note:
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Expression detected by immunofluorescence.
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Specimen
3B:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Mutant Allele(s):
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Epha4tm1Pch/Epha4tm1Pch
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Dent's Fixative
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: segmental spinal nerve |
Present |
Regionally restricted |
3B
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Expression in ventral tibial nerve, but in few or none of the axons in the direction normally followed by the peroneal nerve.
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Specimen
3C:
embryonic day 12.5
(more )
Specimen
3C:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Dent's Fixative
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: segmental spinal nerve |
Present |
Regionally restricted |
3C
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Expression in tibial nerve, divided into several ventral branches, and peroneal nerve, which divides into two branches in the dorsal part of the limb.
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Specimen
3D:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Mutant Allele(s):
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Epha4tm1Pch/Epha4tm1Pch
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Dent's Fixative
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: segmental spinal nerve |
Present |
Regionally restricted |
3D
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Expression in ventral branches of tibial nerve; no expression in the peroneal nerve in most hindlimbs.
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Specimen
3E:
embryonic day 15.5
(more )
Specimen
3E:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Dent's Fixative
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Specimen
3F:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: C57BL/6 * DBA/2)
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Mutant Allele(s):
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Epha4tm1Pch/Epha4tm1Pch
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Dent's Fixative
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: tail segmental spinal nerve |
Present |
Regionally restricted |
3F
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No expression in the peroneal nerve expression in most hindlimbs; expression in an aberran branch from the ventral nerve which contacts the peroneus longus muscle.
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