Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Ephb2tm2Paw
Name:	Eph receptor B2; targeted mutation 2, Tony Pawson
MGI ID:	MGI:2149670
Synonyms:	EphB2ki, EphB2lacZ, EphB2lacZ(kin-), EphB2/LZ, EphB2N2, NuklacZ
Gene:	Ephb2  Location: Chr4:136374850-136563299 bp, - strand  Genetic Position: Chr4, 69.0 cM, cytoband D-E
Alliance:	Ephb2tm2Paw page

Germline Transmission:	Earliest citation of germline transmission: J:34200
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Allele Type:    Targeted (Null/knockout, Reporter) Mutation:    Insertion   Mutation details: Insertion of a LacZ-neomycin resistance cassette in-frame at codon 622. Western blot analysis using a polyclonal Ephb2 antibody did not detect protein in cell surface immunoprecipitates of primary brain cultures from homozygous mutant neonates (P4), however a polyclonal beta-galactosidase antibody did detect a novel 200 kDa protein in these cultures. The authors propose that the mutant allele produces a fusion receptor protein comprised of the extracellular, transmembrane, and juxtamembrane domains (amino acids 1-621) linked to beta-galactosidase, and that this mutated receptor lacks the entire tyrosine kinase catalytic and C-terminal domains (amino acids 622-707). (J:34200)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:	117 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Ephb2 Mutation:	66 strains or lines available

Original:	J:34200 Henkemeyer M, et al., Nuk controls pathfinding of commissural axons in the mammalian central nervous system. Cell. 1996 Jul 12;86(1):35-46
All:	49 reference(s)