Probe:	Bmp4
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS17: apical ectodermal ridge	Strong	Regionally restricted	5A	Strong expression in the posterior and anterior margins, including both the ectoderm, the underlying mesenchyme, the apical ectodermal ridge (AER), and in the progress zone below the AER.
TS17: limb ectoderm	Strong	Regionally restricted	5A	Strong expression in the posterior and anterior margins, including both the ectoderm, the underlying mesenchyme, the apical ectodermal ridge (AER), and in the progress zone below the AER.
TS17: limb mesenchyme	Strong	Regionally restricted	5A	Strong expression in the posterior and anterior margins, including both the ectoderm, the underlying mesenchyme, the apical ectodermal ridge (AER), and in the progress zone below the AER.

Structure	Level	Pattern	Image	Note
TS17: apical ectodermal ridge	Present	Regionally restricted	5B	The strong expression in the posterior margin seen in the wild-type is virtually absent in the mutant. The expression in the apical ectodermal ridge and in the progress zone below it seen in the wild-type are present in the mutant.
TS17: limb	Present	Regionally restricted	5B	The strong expression in the posterior margin seen in the wild-type is virtually absent in the mutant. The expression in the apical ectodermal ridge and in the progress zone below it seen in the wild-type are present in the mutant.
TS17: limb mesenchyme	Present	Regionally restricted	5B	The strong expression in the posterior margin seen in the wild-type is virtually absent in the mutant. The expression in the apical ectodermal ridge and in the progress zone below it seen in the wild-type are present in the mutant.

Structure	Level	Pattern	Image	Note
TS17: apical ectodermal ridge	Present	Regionally restricted		The strong expression in the posterior margin seen in the wild-type is virtually absent in the mutant. The expression in the apical ectodermal ridge and in the progress zone below it seen in the wild-type are present in the mutant.
TS17: limb	Present	Regionally restricted		The strong expression in the posterior margin seen in the wild-type is virtually absent in the mutant. The expression in the apical ectodermal ridge and in the progress zone below it seen in the wild-type are present in the mutant.
TS17: limb mesenchyme	Present	Regionally restricted		The strong expression in the posterior margin seen in the wild-type is virtually absent in the mutant. The expression in the apical ectodermal ridge and in the progress zone below it seen in the wild-type are present in the mutant.

Structure	Level	Pattern	Image	Note
TS19: apical ectodermal ridge	Strong	Regionally restricted	5C	Expression is present in the anterior and posterior marginal zone, including the ectoderm and the mesenchyme; in the AER and the progress zone below it; and in low levels in domains in the proximal bud that appear to correspond to the developing joints.
TS19: limb ectoderm	Strong	Regionally restricted	5C	Expression is present in the anterior and posterior marginal zone, including the ectoderm and the mesenchyme; in the AER and the progress zone below it; and in low levels in domains in the proximal bud that appear to correspond to the developing joints.
TS19: limb mesenchyme	Strong	Regionally restricted	5C	Expression is present in the anterior and posterior marginal zone, including the ectoderm and the mesenchyme; in the AER and the progress zone below it; and in low levels in domains in the proximal bud that appear to correspond to the developing joints.

Structure	Level	Pattern	Image	Note
TS19: apical ectodermal ridge	Present	Regionally restricted	5D	As in wild type, expression is present in the AER and the progress zone below it. However, expression is absent in the anterior and posterior limb bud and is reduced in some of the expression domains that may correspond to developing joints.
TS19: limb	Present	Regionally restricted	5D	As in wild type, expression is present in the AER and the progress zone below it. However, expression is absent in the anterior and posterior limb bud and is reduced in some of the expression domains that may correspond to developing joints.
TS19: limb mesenchyme	Present	Regionally restricted	5D	As in wild type, expression is present in the AER and the progress zone below it. However, expression is absent in the anterior and posterior limb bud and is reduced in some of the expression domains that may correspond to developing joints.

Structure	Level	Pattern	Image	Note
TS19: apical ectodermal ridge	Present	Regionally restricted		As in wild type, expression is present in the AER and in the progress zone below it. However, expression is absent in the anterior and posterior limb bud and is reduced in some of the expression domains that may correspond to developing joints.
TS19: limb	Present	Regionally restricted		As in wild type, expression is present in the AER and in the progress zone below it. However, expression is absent in the anterior and posterior limb bud and is reduced in some of the expression domains that may correspond to developing joints.
TS19: limb mesenchyme	Present	Regionally restricted		As in wild type, expression is present in the AER and in the progress zone below it. However, expression is absent in the anterior and posterior limb bud and is reduced in some of the expression domains that may correspond to developing joints.