Gene Expression Data
Assay Details
Assay
|
Reference:
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J:67151
Kruger M, et al., Sonic hedgehog is a survival factor for hypaxial muscles during mouse development. Development. 2001 Mar;128(5):743-52
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2149560
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Gene symbol:
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Myod1
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Gene name:
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myogenic differentiation 1
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Probe:
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MyoD probe9
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
2A:
embryonic day 10.5
(more )
Specimen
2A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
|
whole mount
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Specimen
2D:
(close )
Genetic Background:
|
involves: 101 * C3H
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Mutant Allele(s):
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ShhDsh/ShhDsh
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Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: forelimb bud mesenchyme |
Present |
Regionally restricted |
2D
|
Expression was present in the muscle precursors, but it was slightly delayed relative to wild type.
|
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Shhtm1Chg/Shhtm1Chg
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Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: forelimb bud mesenchyme |
Present |
Regionally restricted |
|
Expression was present in the muscle precursors, but it was slightly delayed relative to wild type.
|
Specimen
2G:
embryonic day 10.5
(more )
Note: This specimen was stained as a whole mount, embedded in gelatin, and cut on a vibratome.
Specimen
2G:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Embedding:
|
Gelatin
|
Note:
|
This specimen was stained as a whole mount, embedded in gelatin, and cut on a vibratome.
|
Specimen
2J:
embryonic day 10.5; ShhDsh/ ShhDsh
(more )
Note: This specimen was stained as a whole mount, embedded in gelatin, and cut on a vibratome.
Specimen
2J:
(close )
Genetic Background:
|
involves: 101 * C3H
|
Mutant Allele(s):
|
ShhDsh/ShhDsh
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Embedding:
|
Gelatin
|
Note:
|
This specimen was stained as a whole mount, embedded in gelatin, and cut on a vibratome.
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: trunk dermomyotome |
Strong |
Regionally restricted |
2J
|
Expression was present in the hypaxial domain but absent from the epaxial domain.
|
TS17: forelimb bud mesenchyme |
Present |
Regionally restricted |
2J
|
Expression was present in the muscle precursors, but it was slightly delayed relative to wild type.
|
Specimen
not shown:
embryonic day 10.5; Shhtm1Chg/ Shhtm1Chg
(more )
Note: This specimen was stained as a whole mount, embedded in gelatin, and cut on a vibratome.
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Shhtm1Chg/Shhtm1Chg
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Embedding:
|
Gelatin
|
Note:
|
This specimen was stained as a whole mount, embedded in gelatin, and cut on a vibratome.
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: trunk dermomyotome |
Strong |
Regionally restricted |
|
Expression was present in the hypaxial domain but absent from the epaxial domain.
|
TS17: forelimb bud mesenchyme |
Present |
Regionally restricted |
|
Expression was present in the muscle precursors, but it was slightly delayed relative to wild type.
|
Specimen
3F:
embryonic day 10.5; ShhDsh/ ShhDsh
(more )
Note: The somites of mutant embryos display significant malformations. In the dorsomedial region next to the neural tube, no epithelium was discernible. This specimen was embedded in gelatin and cut on a vibratome.
Specimen
3F:
(close )
Genetic Background:
|
involves: 101 * C3H
|
Mutant Allele(s):
|
ShhDsh/ShhDsh
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Embedding:
|
Gelatin
|
Note:
|
The somites of mutant embryos display significant malformations. In the dorsomedial region next to the neural tube, no epithelium was discernible. This specimen was embedded in gelatin and cut on a vibratome.
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: trunk dermomyotome |
Present |
Regionally restricted |
3F
|
Expression was present in the hypaxial domain but absent from the epaxial domain.
|
Specimen
not shown:
embryonic day 10.5; Shhtm1Chg/ Shhtm1Chg
(more )
Note: The somites of mutant embryos display significant malformations. In the dorsomedial region next to the neural tube, no epithelium was discernible. This specimen was embedded in gelatin and cut on a vibratome.
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Shhtm1Chg/Shhtm1Chg
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Embedding:
|
Gelatin
|
Note:
|
The somites of mutant embryos display significant malformations. In the dorsomedial region next to the neural tube, no epithelium was discernible. This specimen was embedded in gelatin and cut on a vibratome.
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: trunk dermomyotome |
Present |
Regionally restricted |
|
Expression was present in the hypaxial domain but absent from the epaxial domain.
|
|