Gene Expression Data
Assay Details
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Assay
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Reference:
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J:56583
Ring BZ, et al., Regulation of mouse lens fiber cell development and differentiation by the Maf gene. Development. 2000 Jan;127(2):307-17
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2149420
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Gene symbol:
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Hspb8
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Gene name:
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heat shock protein 8
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Antibody:
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alpha-crystallin antibody
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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Antibody recognizes all crystallins within the alpha subtype; all possibilities (Cryaa, Cryab, Cryac) have been annotated.
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Results
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Specimen
4A E12 left:
embryonic day 12.0; Maftm1Gsb/Maf +
(more  )
Specimen
4A E12 left:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Maftm1Gsb/Maf+
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
4A E12 right:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Maftm1Gsb/Maftm1Gsb
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
4A E15.5 left:
embryonic day 15.5; Maftm1Gsb/Maf +
(more )
Specimen
4A E15.5 left:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Maftm1Gsb/Maf+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
4A E15.5 right:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Maftm1Gsb/Maftm1Gsb
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lens |
Weak |
Spotted |
4A E15.5 alpha-cryst. right
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Greatly reduced expression, in a unusual speckled pattern; more striking in fiber cells than in epithelial cells.
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Analysis Tools