Gene Expression Data
Assay Details
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Assay
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Reference:
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J:45780
Tiret L, et al., Increased apoptosis of motoneurons and altered somatotopic maps in the brachial spinal cord of Hoxc-8-deficient mice. Development. 1998 Jan;125(2):279-91
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2149091
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Gene symbol:
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Isl2
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Gene name:
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insulin related protein 2 (islet 2)
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Antibody:
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anti-C-Isl-1 protein (K5)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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Antibody used detects both Isl1 and Isl2; both genes have been annotated.
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Results
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Specimen
2F:
embryonic day 13.5; Hoxc8tm1Phb/Hoxc8 +
(more  )
Note: Double labeled: brown - Isl1 and Isl2; turquoise/blue - Hoxc8 (assay MGI:3699628).
Specimen
2F:
(close  )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8+
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Isl1 and Isl2; turquoise/blue - Hoxc8 (assay MGI:3699628).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Regionally restricted |
2F
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Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 8.
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Specimen
3I - C6:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8tm1Phb
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Isl1 and Isl2; turquoise/blue - Hoxc8 (assay MGI:3699628).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Single cells |
3I
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Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 6.
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Specimen
3J - C7:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8tm1Phb
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Isl1 and Isl2; turquoise/blue - Hoxc8 (assay MGI:3699628).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Single cells |
3J
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Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 7.
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Specimen
3K - C8 LMC:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8tm1Phb
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Isl1 and Isl2; turquoise/blue - Hoxc8 (assay MGI:3699628).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Single cells |
3K
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Expression was detected in motor neurons of the lateral motor column. The specimen was from the level of the cervical vertebra 8.
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Specimen
3L - C8 MMC:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8tm1Phb
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Isl1 and Isl2; turquoise/blue - Hoxc8 (assay MGI:3699628).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Single cells |
3L
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Expression was detected in motor neurons of the medial motor column. The specimen was from the level of the cervical vertebra 8.
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Specimen
3M - C6:
embryonic day 13.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: brown - Isl1 and Isl2; turquoise/blue - Hoxc8 (assay MGI:3699628).
Specimen
3M - C6:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8+
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
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Note:
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Double labeled: brown - Isl1 and Isl2; turquoise/blue - Hoxc8 (assay MGI:3699628).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Single cells |
3M
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Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 6.
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Specimen
3N - C7:
embryonic day 13.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: brown - Isl1 and Isl2; turquoise/blue - Hoxc8 (assay MGI:3699628).
Specimen
3N - C7:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8+
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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|
Note:
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Double labeled: brown - Isl1 and Isl2; turquoise/blue - Hoxc8 (assay MGI:3699628).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Single cells |
3N
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Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 7.
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Specimen
3O - C8 LMC:
embryonic day 13.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: brown - Isl1 and Isl2; turquoise/blue - Hoxc8 (assay MGI:3699628).
Specimen
3O - C8 LMC:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8+
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
|
Note:
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Double labeled: brown - Isl1 and Isl2; turquoise/blue - Hoxc8 (assay MGI:3699628).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Single cells |
3O
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Expression was detected in motor neurons of the lateral motor column. The specimen was from the level of the cervical vertebra 8.
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Specimen
3P - C8 MMC:
embryonic day 13.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: brown - Isl1 and Isl2; turquoise/blue - Hoxc8 (assay MGI:3699628).
Specimen
3P - C8 MMC:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8+
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
|
|
Note:
|
Double labeled: brown - Isl1 and Isl2; turquoise/blue - Hoxc8 (assay MGI:3699628).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Single cells |
3P
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Expression was detected in motor neurons of the medial motor column. The specimen was from the level of the cervical vertebra 8.
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Specimen
4A:
embryonic day 12.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Specimen
4A:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8+
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Single cells |
4A
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Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 8.
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Specimen
4B:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8tm1Phb
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Single cells |
4B
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Expression was detected in motor neurons, and the number did not differ from the heterozygous embryo. The specimen was from the level of the cervical vertebra 8.
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Specimen
4M:
embryonic day 15.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Specimen
4M:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8+
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: spinal cord |
Present |
Single cells |
4M
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Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 8.
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Specimen
4N:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8tm1Phb
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: spinal cord |
Present |
Single cells |
4N
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Expression was detected in motor neurons, but there were fewer motor neurons in the mutant. The specimen was from the level of the cervical vertebra 8.
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