Gene Expression Data
Assay Details
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Assay
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Reference:
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J:45780
Tiret L, et al., Increased apoptosis of motoneurons and altered somatotopic maps in the brachial spinal cord of Hoxc-8-deficient mice. Development. 1998 Jan;125(2):279-91
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2149088
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Gene symbol:
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Hoxc8
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Gene name:
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homeobox C8
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Antibody:
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BabCO #MMX-266R
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
3A - C6:
(close  )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8+
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Hoxc8; red - Isl1 (assay MGI:3700202) and Isl2 (assay MGI:3700203).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Single cells |
3A
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The specimen was from the level of the cervical vertebra 6. At this level, expression was restricted to interneurons and/or glial cells.
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Specimen
3B - C7:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8+
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Hoxc8; red - Isl1 (assay MGI:3700202) and Isl2 (assay MGI:3700203).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Single cells |
3B
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The specimen was from the level of the cervical vertebra 7. At the rostral part of this level, expression was detected in a mosaic population of motor neurons. In the medial and caudal parts of this level, expression was in 100% of the motor neurons.
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Specimen
3C - C8:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8+
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Hoxc8; red - Isl1 (assay MGI:3700202) and Isl2 (assay MGI:3700203).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Single cells |
3C
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The specimen was from the level of the cervical vertebra 8. At this level, expression was detected in 100% of somatic motor neurons.
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Specimen
3D - T1:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8+
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Hoxc8; red - Isl1 (assay MGI:3700202) and Isl2 (assay MGI:3700203).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Single cells |
3D
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The specimen was from the level of the thoracic vertebra 1. At this level, expression was detected in 100% of somatic motor neurons.
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Specimen
3E - C6:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8+
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
|
|
Note:
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Double labeled: green - Hoxc8; red - Isl1 (assay MGI:3700202) and Isl2 (assay MGI:3700203).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: spinal cord |
Present |
Single cells |
3E
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The specimen was from the level of the cervical vertebra 6. At this level, expression was restricted to interneurons and/or glial cells.
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Specimen
3F - C7:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8+
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Hoxc8; red - Isl1 (assay MGI:3700202) and Isl2 (assay MGI:3700203).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: spinal cord |
Present |
Single cells |
3F
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The specimen was from the level of the cervical vertebra 7. At the rostral part of this level, expression was detected in a mosaic population of motor neurons. In the medial and caudal parts of this level, expression was in 100% of the motor neurons.
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Specimen
3G - C8 LMC:
embryonic day 14.5; Hoxc8tm1Phb/Hoxc8 +
(more  )
Note: Double labeled: green - Hoxc8; red - Isl1 (assay MGI:3700202) and Isl2 (assay MGI:3700203).
Specimen
3G - C8 LMC:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8+
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
|
Embedding:
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Cryosection
|
|
Note:
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Double labeled: green - Hoxc8; red - Isl1 (assay MGI:3700202) and Isl2 (assay MGI:3700203).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: spinal cord |
Present |
Single cells |
3G
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The specimen was from the level of the cervical vertebra 8. At this level, expression was detected in 100% of somatic motor neurons in the lateral motor column.
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Specimen
3H - C8 MMC:
embryonic day 14.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: green - Hoxc8; red - Isl1 (assay MGI:3700202) and Isl2 (assay MGI:3700203).
Specimen
3H - C8 MMC:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8+
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
|
Note:
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Double labeled: green - Hoxc8; red - Isl1 (assay MGI:3700202) and Isl2 (assay MGI:3700203).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: spinal cord |
Present |
Single cells |
3H
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The specimen was from the level of the cervical vertebra 8. At this level, expression was detected in 100% of somatic motor neurons of the medial motor column.
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Analysis Tools